RASA1, Polyclonal Antibody

Ras GTPase-activating protein 1; GAP; GTPase-activating protein; RasGAP; Ras p21 protein activator; p120GAP; RASA1; GAP; RASA

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

>95%,Protein G purified

Liquid

Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-RASA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1.

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

Western Blot
Positive WB detected in:Hela whole cell lysate,Mouse brain tissue
All lanes: RASA1 antibody at 3.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 117,101,59,102 kDa
Observed band size: 117 kDa

Immunofluorescent analysis of PC3 cells using MBS7006455 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

101,546 Da

RAS p21 protein activator 1

ras GTPase-activating protein 1

Ras GTPase-activating protein 1

GAP; RASA; GAP; GTPase-activating protein; RasGAP  [Similar Products]

RASA1_HUMAN

The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the s of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not ***** tissues. [provided by RefSeq, May 2012]

RASA1: a GTPase activator for normal RAS p21 but not its oncogenic counterpart, converting it to the putatively inactive GDP-bound state. Acting as a suppressor of RAS function, it enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the s of either protein are associated with basal cell carcinomas. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; GAPs; GAPs, Ras; Oncoprotein

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: cytoplasm; cytosol; ruffle

Molecular Function: glycoprotein binding; GTPase activator activity; GTPase binding; potassium channel inhibitor activity; protein binding; receptor binding

Biological Process: activation of MAPKK activity; axon guidance; blood vessel morphogenesis; cytokinesis after mitosis; embryonic development; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; negative regulation of cell adhesion; negative regulation of cell-matrix adhesion; negative regulation of neuron apoptosis; negative regulation of Ras protein signal transduction; nerve growth factor receptor signaling pathway; positive regulation of GTPase activity; Ras protein signal transduction; regulation of actin filament polymerization; regulation of cell shape; regulation of RNA metabolic process; signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway; vasculogenesis

Disease: Basal Cell Carcinoma, Susceptibility To, 1; Capillary Malformation-arteriovenous Malformation; Parkes Weber Syndrome

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