Product Name
ATP7B, siRNA
Full Product Name
ATP7B siRNA (Mouse)
Product Synonym Names
WND; Copper-transporting ATPase 2; Copper pump 2; Wilson disease-associated protein homolog
Product Gene Name
ATP7B sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q64446
Specificity
ATP7B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ATP7B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ATP7B sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ATP7B sirna
siRNA to inhibit ATP7B expression using RNA interference
Applications Tested/Suitable for ATP7B sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ATP7B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_031537.2
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NCBI GenBank Nucleotide #
NM_007511.2
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UniProt Primary Accession #
Q64446
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UniProt Secondary Accession #
B1AQ56[Other Products]
UniProt Related Accession #
Q64446[Other Products]
Molecular Weight
157,190 Da
NCBI Official Full Name
copper-transporting ATPase 2
NCBI Official Synonym Full Names
ATPase, Cu++ transporting, beta polypeptide
NCBI Official Symbol
Atp7b [Similar Products]
NCBI Official Synonym Symbols
tx; WND; Atp7a
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NCBI Protein Information
copper-transporting ATPase 2
UniProt Protein Name
Copper-transporting ATPase 2
UniProt Synonym Protein Names
Copper pump 2; Wilson disease-associated protein homolog
UniProt Gene Name
Atp7b [Similar Products]
UniProt Synonym Gene Names
Wnd [Similar Products]
UniProt Entry Name
ATP7B_MOUSE
UniProt Comments for ATP7B
ATP7B: Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. Monomer. Interacts with COMMD1/MURR1. Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Transporter; Transporter, ion channel; Vesicle; EC 3.6.3.54; Hydrolase; Membrane protein, multi-pass
Cellular Component: Golgi apparatus; tight junction; membrane; cell; perinuclear region of cytoplasm; basolateral plasma membrane; cytoplasmic membrane-bound vesicle; late endosome; integral to membrane; plasma membrane; trans-Golgi network; intracellular
Molecular Function: copper ion transmembrane transporter activity; copper ion binding; zinc ion binding; hydrolase activity; metal ion binding; nucleotide binding; copper-transporting ATPase activity; copper-exporting ATPase activity; cation-transporting ATPase activity; ATP binding
Biological Process: lactation; cellular copper ion homeostasis; metal ion transport; response to copper ion; transport; sequestering of calcium ion; cellular zinc ion homeostasis; copper ion import; ion transport; copper ion transport; copper ion export; cation transport; intracellular copper ion transport
Research Articles on ATP7B
1. DKWSLLL sequence is essential for ATP7b sorting at the TGN, transport from the TGN to the PM, endocytosis, and recycling to the TGN and PM.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
