Full Product Name
IGF-1 Antibody: Dylight 594
Product Synonym Names
Rabbit Anti-Human IGF-1 Polyclonal; Insulin-like growth factor 1 Antibody; Igf1 Antibody; IGF-I Antibody; Insulin like growth factor 1 (somatomedin C) Antibody; IGF1_HUMAN Antibody; Somatomedin-C Antibody; MGF Antibody; OTTHUMP00000195083 Antibody; Somatomedia C Antibody; Mechano growth factor Antibody; Insulin like growth factor 1 Antibody; Insulin like growth factor IA Antibody; IGF IB Antibody; IBP1 Antibody; IGF1 Antibody; Insulin-like growth factor I Antibody; IGF IA Antibody; Somatomedin C Antibody; IGF1A Antibody; IGFI Antibody; IGFIA Antibody; IGF I Antibody; Insulin like growth factor IB Antibody; IGF 1 Antibody; IGF I Antibody
Product Gene Name
anti-IGF-1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P05019
Species Reactivity
Human, Mouse
Specificity
Detects ~25 kDa.
Purity/Purification
Peptide Affinity Purified
Form/Format
PBS, 50% Glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Synthetic peptide from the mid-protein of Human IGF-1
Cellular Localization
Secreted
Certificate of Analysis
A 1:1000 dilution was sufficient for detection of IGF-1 in 15 ug of mouse liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, Förster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-IGF-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-IGF-1 antibody
Cell Signaling; Growth Factors; Insulin and Insulin-Like; Cancer
Applications Tested/Suitable for anti-IGF-1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-IGF-1 antibody
WB: 1:1000
ICC/IF: 1:100
IHC: 1:50
Immunocytochemistry (ICC) of anti-IGF-1 antibody
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IGF-1 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IGF-1 Polyclonal Antibody. Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-IGF-1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IGF-1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~21.8 kDa.
NCBI/Uniprot data below describe general gene information for IGF-1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001104755.1
[Other Products]
NCBI GenBank Nucleotide #
NP_001104755.1
[Other Products]
UniProt Primary Accession #
P05019
[Other Products]
UniProt Related Accession #
P05019[Other Products]
NCBI Official Full Name
insulin-like growth factor I isoform 3 preproprotein
NCBI Official Synonym Full Names
insulin like growth factor 1
NCBI Official Symbol
IGF1 [Similar Products]
NCBI Official Synonym Symbols
IGF; MGF; IGFI; IGF-I
[Similar Products]
NCBI Protein Information
insulin-like growth factor I
UniProt Protein Name
Insulin-like growth factor I
UniProt Synonym Protein Names
Mechano growth factor; MGF; Somatomedin-C
UniProt Gene Name
IGF1 [Similar Products]
UniProt Synonym Gene Names
IBP1; IGF-I; MGF [Similar Products]
UniProt Entry Name
IGF1_HUMAN
NCBI Summary for IGF-1
The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]
UniProt Comments for IGF-1
IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted
Chromosomal Location of Human Ortholog: 12q23.2
Cellular Component: insulin-like growth factor binding protein complex; extracellular space; plasma membrane; extracellular region
Molecular Function: integrin binding; insulin-like growth factor receptor binding; protein binding; growth factor activity; hormone activity; insulin receptor binding
Biological Process: muscle development; positive regulation of transcription, DNA-dependent; chondroitin sulfate proteoglycan biosynthetic process; glycolate metabolic process; exocrine pancreas development; water homeostasis; positive regulation of glucose import; positive regulation of fibroblast proliferation; proteoglycan biosynthetic process; inner ear development; positive regulation of DNA binding; muscle hypertrophy; platelet activation; positive regulation of mitosis; positive regulation of protein import into nucleus, translocation; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; insulin-like growth factor receptor signaling pathway; regulation of gene expression; response to heat; positive regulation of transcription from RNA polymerase II promoter; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of smooth muscle cell proliferation; myoblast proliferation; positive regulation of glycogen biosynthetic process; positive regulation of activated T cell proliferation; positive regulation of smooth muscle cell migration; signal transduction; negative regulation of cell proliferation; glial cell differentiation; platelet degranulation; positive regulation of MAPKKK cascade; mammary gland development; positive regulation of cell proliferation; DNA replication; skeletal development; positive regulation of granule cell precursor proliferation; phosphoinositide-mediated signaling; multicellular organism growth; regulation of multicellular organism growth; myotube cell development; satellite cell compartment self-renewal involved in skeletal muscle regeneration; myoblast differentiation; positive regulation of osteoblast differentiation; cell proliferation; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of glycolysis; Ras protein signal transduction; blood vessel remodeling; positive regulation of Ras protein signal transduction; blood coagulation; cell motility; positive regulation of DNA replication
Disease: Insulin-like Growth Factor I Deficiency
Research Articles on IGF-1
1. study of association of IGFBP3 and IGF1 polymorphisms with susceptibility to colorectal cancer [Meta-analysis]
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