AICDA / AID, Polyclonal Antibody

Anti-AICDA / AID Antibody (C-Terminus) IHC-plus; AICDA; AID; ARP2; CDA2; Cytidine aminohydrolase; HIGM2; Human AICDA; AID

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

peptide corresponding to 14 amino acids near the carboxy-terminus of human AID

Immunoaffinity Purified

PBS, 0.02% sodium azide.

1 mg/ml (lot specific)

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Small volumes of anti-AICDA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Activation-induced cytidine deaminase (AICDA) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. AICDA is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms.

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)

ICC (10 ug/ml), IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibo...

Anti-AICDA antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-AICDA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence of AID in Ramos cells with AID antibody at 20 ug/ml.

Immunocytochemistry of AID in Ramos cells with Ramos antibody at 10 ug/ml.

Western blot of AID in Ramos whole cell lysate with AID antibody at 2 ug/ml in either the (A) absence or (B) presence of blocking peptide.

22,614 Da

activation-induced cytidine deaminase

single-stranded DNA cytosine deaminase; cytidine aminohydrolase; epididymis secretory protein Li 284; integrated into Burkitt's lymphoma cell line Ramos

Single-stranded DNA cytosine deaminase

AID  [Similar Products]

AICDA_HUMAN

This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Feb 2009]

AID: Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class- switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Strongly expressed in lymph nodes and tonsils. Belongs to the cytidine and deoxycytidylate deaminase family.

Protein type: EC 3.5.4.38; Hydrolase

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytoplasm; exosome (RNase complex); nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin protein ligase binding; cytidine deaminase activity

Biological Process: somatic diversification of immunoglobulins; B cell differentiation; somatic hypermutation of immunoglobulin genes; isotype switching; cytidine deamination; mRNA processing

Disease: Immunodeficiency With Hyper-igm, Type 2

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