Full Product Name
Goat anti-FUT2 Antibody
Product Synonym Names
FUT2; fucosyltransferase 2 (secretor status included); B12QTL1; SE; SEC2; Se2; sej; GDP-L-fucose: beta-D-galactoside 2-alpha-L-fucosyltransferase 2; alpha (1,2) fucosyltransferase; alpha(1,2)FT 2; alpha(1,2)FT2; galactoside 2-alpha-L-fucosyltransferase 2; FUT2 antibody; fucosyltransferase 2 (secretor status included) antibody; B12QTL1 antibody; SE antibody; SEC2 antibody; Se2 antibody; sej antibody; GDP-L-fucose: beta-D-galactoside 2-alpha-L-fucosyltransferase 2 antibody; alpha (1; 2) fucosyltransferase antibody; alpha(1; 2)FT2 antibody; galactoside 2-alpha-L-fucosyltransferase 2 antibody
Product Gene Name
anti-FUT2 antibody
[Similar Products]
Antibody/Peptide Pairs
FUT2 peptide (MBS428123) is used for blocking the activity of FUT2 antibody (MBS423190)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
HVQQRLAKIQ AMWE
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-HVQQRLAKIQAMWE, from the internal region (near N Terminus) of the protein sequence according to NP_000502.4.
Epitope
Internal region (near N Terminus)
Note
Reported variants represent identical protein: NP_001091107.1, NP_000502.4
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-FUT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-FUT2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-FUT2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 37kDa band observed in Human Liver lysates (calculated MW of 39. 0kDa according to NP_000502.4. Recommended concentration: 1-3ug/ml.
Western Blot (WB) of anti-FUT2 antibody
(1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NCBI/Uniprot data below describe general gene information for FUT2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000502.4
[Other Products]
NCBI GenBank Nucleotide #
NM_000511.5
[Other Products]
UniProt Secondary Accession #
Q0VAG5; Q14338; Q5D0G2[Other Products]
UniProt Related Accession #
Q10981[Other Products]
Molecular Weight
37,740 Da
NCBI Official Full Name
galactoside 2-alpha-L-fucosyltransferase 2
NCBI Official Synonym Full Names
fucosyltransferase 2
NCBI Official Symbol
FUT2 [Similar Products]
NCBI Official Synonym Symbols
SE; Se2; sej; SEC2; B12QTL1
[Similar Products]
NCBI Protein Information
galactoside 2-alpha-L-fucosyltransferase 2
UniProt Protein Name
Galactoside 2-alpha-L-fucosyltransferase 2
UniProt Synonym Protein Names
Alpha(1,2)FT 2; Fucosyltransferase 2; GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 2; SE2; Secretor blood group alpha-2-fucosyltransferase; Secretor factor; Se
Protein Family
Fucosyltransferase
UniProt Gene Name
FUT2 [Similar Products]
UniProt Synonym Gene Names
SEC2; Se [Similar Products]
UniProt Entry Name
FUT2_HUMAN
NCBI Summary for FUT2
The protein encoded by this gene is a Golgi stack membrane protein that is involved in the creation of a precursor of the H antigen, which is required for the final step in the soluble A and B antigen synthesis pathway. This gene is one of two encoding the galactoside 2-L-fucosyltransferase enzyme. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for FUT2
FUT2: Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values. Belongs to the glycosyltransferase 11 family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Membrane protein, integral; EC 2.4.1.69; Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Transferase
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: Golgi apparatus; integral to membrane
Molecular Function: fucosyltransferase activity; galactoside 2-alpha-L-fucosyltransferase activity
Biological Process: carbohydrate metabolic process; L-fucose catabolic process; protein amino acid glycosylation
Disease: Vitamin B12 Plasma Level Quantitative Trait Locus 1
Research Articles on FUT2
1. In more than 1000 patients with pancreatitis, we confirmed that FUT2 non-secretor status and blood type B are also disease risk factors, with a greater than twofold OR for blood type B compared with blood type O.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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