GluN2A/NR2A, Monoclonal Antibody

NMDA 2A; NMDAR2A; NMDAR 2A; NMDA Receptor 2A; Glutamate Receptor; GRIN2A; Glutamate [NMDA] Receptor subunit epsilon-1; Glutamate receptor ionotropic N methyl D aspartate 2A; HNR2A; N methyl D aspartate receptor channel; subunit epsilon 1

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

S327-95

Mouse

Protein G Purified

1mg/mL (lot specific)

-20 degree C

Small volumes of anti-Grin2a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background Info: Detects ~170kDa. Does not react with NR2B

Scientific Background: N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D).

Cell Signaling, Neuroscience

Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC)

1:1000 (WB)

Western Blot analysis of Monkey COS cells transfected with GFP-tagged NR2A showing detection of ~170 kDa GluN2A/NR2A protein using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327-95. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NR2A. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa. Other Band(s): 100 kDa.

165,469 Da[Similar Products]

glutamate receptor, ionotropic, N-methyl D-aspartate 2A

glutamate receptor ionotropic, NMDA 2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor subunit 2A; glutamate [NMDA] receptor subunit epsilon-1

Glutamate receptor ionotropic, NMDA 2A

GluN2A; NMDAR2A; NR2A  [Similar Products]

NMDE1_RAT

may play a role in synaptic transmission, learning and memory [RGD, Feb 2006]

NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission.

Protein type: Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; membrane; endoplasmic reticulum; postsynaptic density; dendritic spine; terminal button; synapse; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: protein dimerization activity; voltage-gated cation channel activity; neurotransmitter binding; glutamate receptor binding; zinc ion binding; calcium channel activity; protein kinase binding; ATPase binding; protein binding; extracellular-glutamate-gated ion channel activity; glutamate binding; ionotropic glutamate receptor activity; protein heterodimerization activity; N-methyl-D-aspartate selective glutamate receptor activity; protein complex binding; cell adhesion molecule binding; cation channel activity; receptor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; startle response; positive regulation of apoptosis; rhythmic process; regulation of synaptic plasticity; response to other organism; sensory perception of pain; response to organic cyclic substance; dopamine metabolic process; response to carbohydrate stimulus; synaptic transmission; protein localization; learning and/or memory; calcium ion transport; response to methylmercury; response to wounding; visual learning; locomotion; protein tetramerization; cation transport; serotonin metabolic process; regulation of synaptic transmission; response to drug; synaptic transmission, glutamatergic; response to light stimulus; regulation of action potential; hippocampus development; response to amphetamine; learning; response to cocaine; sleep; memory; response to manganese ion; detection of mechanical stimulus involved in sensory perception of pain; regulation of membrane potential; regulation of postsynaptic membrane potential; response to ethanol; neurogenesis; spinal cord development; ionotropic glutamate receptor signaling pathway; directional locomotion; regulation of sensory perception of pain; cerebral cortex development; regulation of excitatory postsynaptic membrane potential; response to calcium ion; negative regulation of protein catabolic process; response to amine stimulus

1. Teng H.J., et al. (2010) PLoS ONE. 5: e13342.

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