LAMC1, Polyclonal Antibody

Laminin subunit gamma-1 [Precursor]; Laminin B2 chain; LAMC1; LAMB2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total LAMC1 protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-LAMC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.Kallunki T., J. Biol. Chem. 266:221-228(1991).Pikkarainen T., J. Biol. Chem. 263:6751-6758(1988).Fukushima Y., Cytogenet. Cell Genet. 48:137-141(1988).

Total protein Ab

Western Blot (WB)

Western blotting: 1:500~1:3000

Western blot analysis of extracts from HUVEC cells, using LAMC1 antibody.

177,603 Da

laminin, gamma 1 (formerly LAMB2)

laminin subunit gamma-1

Laminin subunit gamma-1

LAMB2; S-LAM gamma  [Similar Products]

LAMC1_HUMAN

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. [provided by RefSeq, Aug 2011]

LAMC1: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: extracellular matrix; extracellular space; laminin-1 complex; laminin-10 complex; extracellular region; basement membrane

Molecular Function: extracellular matrix structural constituent; glycosphingolipid binding

Biological Process: extracellular matrix disassembly; axon guidance; extracellular matrix organization and biogenesis; cell migration; hemidesmosome assembly; protein complex assembly; cell adhesion; positive regulation of epithelial cell proliferation; endoderm development

Disease: Pelvic Organ Prolapse, Susceptibility To

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