Full Product Name
GBE1 siRNA (Human)
Product Synonym Names
1.4-alpha-glucan-branching enzyme; Brancher enzyme; Glycogen-branching enzyme
Product Gene Name
GBE1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q04446
Specificity
GBE1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GBE1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GBE1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GBE1 sirna
siRNA to inhibit GBE1 expression using RNA interference
Applications Tested/Suitable for GBE1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GBE1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000149.3
[Other Products]
NCBI GenBank Nucleotide #
NM_000158.3
[Other Products]
UniProt Primary Accession #
Q04446
[Other Products]
UniProt Secondary Accession #
Q96EN0; B3KWV3[Other Products]
UniProt Related Accession #
Q04446[Other Products]
Molecular Weight
80,474 Da
NCBI Official Full Name
1,4-alpha-glucan-branching enzyme
NCBI Official Synonym Full Names
glucan (1,4-alpha-), branching enzyme 1
NCBI Official Symbol
GBE1 [Similar Products]
NCBI Official Synonym Symbols
GBE; APBD; GSD4
[Similar Products]
NCBI Protein Information
1,4-alpha-glucan-branching enzyme
UniProt Protein Name
1,4-alpha-glucan-branching enzyme
UniProt Synonym Protein Names
Brancher enzyme; Glycogen-branching enzyme
Protein Family
1,4-alpha-glucan branching enzyme
UniProt Gene Name
GBE1 [Similar Products]
UniProt Entry Name
GLGB_HUMAN
NCBI Summary for GBE1
The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease). [provided by RefSeq, Jul 2008]
UniProt Comments for GBE1
GBE1: a monomeric enzyme that functions in glycogen symthesis by catalyzing the formation of alpha 1,6- glucosidic linkages.Required for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells. It is most highly expressed in liver and muscle. Deficiency can result in glycogen storage disease IV (Andersen's disease).
Protein type: Carbohydrate Metabolism - starch and sucrose; EC 2.4.1.18; Transferase
Chromosomal Location of Human Ortholog: 3p12.3
Cellular Component: cytosol
Molecular Function: cation binding; 1,4-alpha-glucan branching enzyme activity; hydrolase activity, hydrolyzing O-glycosyl compounds
Biological Process: glycogen metabolic process; generation of precursor metabolites and energy; glycogen biosynthetic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis
Disease: Polyglucosan Body Disease, Adult Form; Glycogen Storage Disease Iv
Research Articles on GBE1
1. GBE1 mutation is found in manifesting heterozygous patients with ***** polyglucosan body disease
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
