Product Name
GluN2A/NR2A, Monoclonal Antibody
Full Product Name
GluN2A/NR2A Antibody, Clone S327A-38: Dylight 350
Product Synonym Names
Mouse Anti-Rat GluN2A/NR2A Monoclonal IgG2b; GluN2A/NR2A Antibody: Dylight 350; NMDA 2A Antibody; NMDAR2A Antibody; NMDAR 2A Antibody; NMDA Receptor 2A Antibody; Glutamate Receptor Antibody; GRIN2A Antibody; Glutamate [NMDA] Receptor subunit epsilon-1 Antibody; Glutamate receptor ionotropic N methyl D aspartate 2A Antibody; HNR2A Antibody; N methyl D aspartate receptor channel Antibody; subunit epsilon 1 Antibody; N Methyl D Aspartate Receptor Subtype 2A Antibody; N methyl D aspartate receptor subunit 2A Antibody; NMDA receptor subtype 2A Antibody; NMDA Receptor Type 2A Antibody; OTTHUMP00000160135 Antibody; OTTHUMP00000174531 Antibody
Product Gene Name
anti-GluN2A/NR2A antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q00959
Species Reactivity
Human, Mouse, Rat
Specificity
Detects ~170 kDa. Does not react with NR2B.
Purity/Purification
Protein G Purified
Form/Format
PBS pH 7.4, 50% Glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Fusion protein amino acids 75-325 (extracellular N-terminus) of rat GluN2A/NR2A
Cellular Localization
Cell Membrane; Cell Junction
Certificate of Analysis
1 ug/ml was sufficient for detection of GluN2A/NR2A in 20 ug of COS cells transiently transfected with GFP-tagged NR2A lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, Förster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-GluN2A/NR2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GluN2A/NR2A antibody
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D).
Product Categories/Family for anti-GluN2A/NR2A antibody
Neuroscience; Neurotransmitter Receptors; Glutamate Receptors; NMDA Receptors; Cell Signaling
Applications Tested/Suitable for anti-GluN2A/NR2A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-GluN2A/NR2A antibody
WB: 1:1000
ICC/IF: 1:100
Immunocytochemistry (ICC) of anti-GluN2A/NR2A antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327A-38 at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-GluN2A/NR2A Antibody. (C) Composite. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluN2A/NR2A Antibody. (D) Composite.
Western Blot (WB) of anti-GluN2A/NR2A antibody
Western Blot analysis of Monkey COS cells transfected with GFP-tagged NR2A showing detection of ~170 kDa GluN2A/NR2A protein using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327A-38 at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa.
NCBI/Uniprot data below describe general gene information for GluN2A/NR2A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036705.3
[Other Products]
NCBI GenBank Nucleotide #
NP_036705.3
[Other Products]
UniProt Primary Accession #
Q00959
[Other Products]
UniProt Related Accession #
Q00959[Other Products]
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 2A
NCBI Official Synonym Full Names
glutamate ionotropic receptor NMDA type subunit 2A
NCBI Official Symbol
Grin2a [Similar Products]
NCBI Official Synonym Symbols
NR2A; GluN2A; NMDAR2A
[Similar Products]
NCBI Protein Information
glutamate receptor ionotropic, NMDA 2A
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 2A
UniProt Synonym Protein Names
Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A
UniProt Gene Name
Grin2a [Similar Products]
UniProt Synonym Gene Names
GluN2A; NMDAR2A; NR2A [Similar Products]
UniProt Entry Name
NMDE1_RAT
NCBI Summary for GluN2A/NR2A
may play a role in synaptic transmission, learning and memory [RGD, Feb 2006]
UniProt Comments for GluN2A/NR2A
NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission.
Protein type: Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass
Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; membrane; endoplasmic reticulum; postsynaptic density; dendritic spine; terminal button; synapse; cell junction; N-methyl-D-aspartate selective glutamate receptor complex
Molecular Function: voltage-gated cation channel activity; protein dimerization activity; neurotransmitter binding; glutamate receptor binding; zinc ion binding; calcium channel activity; protein kinase binding; ATPase binding; protein binding; glutamate binding; extracellular-glutamate-gated ion channel activity; protein heterodimerization activity; ionotropic glutamate receptor activity; N-methyl-D-aspartate selective glutamate receptor activity; protein complex binding; cell adhesion molecule binding; cation channel activity; receptor binding
Biological Process: regulation of long-term neuronal synaptic plasticity; startle response; positive regulation of apoptosis; rhythmic process; regulation of synaptic plasticity; response to other organism; sensory perception of pain; response to organic cyclic substance; dopamine metabolic process; response to carbohydrate stimulus; synaptic transmission; protein localization; learning and/or memory; calcium ion transport; response to methylmercury; response to wounding; visual learning; locomotion; cation transport; serotonin metabolic process; protein tetramerization; regulation of synaptic transmission; response to drug; synaptic transmission, glutamatergic; response to light stimulus; regulation of action potential; response to amphetamine; hippocampus development; learning; response to cocaine; sleep; memory; response to manganese ion; detection of mechanical stimulus involved in sensory perception of pain; regulation of membrane potential; response to ethanol; regulation of postsynaptic membrane potential; neurogenesis; spinal cord development; directional locomotion; ionotropic glutamate receptor signaling pathway; regulation of sensory perception of pain; cerebral cortex development; negative regulation of protein catabolic process; regulation of excitatory postsynaptic membrane potential; response to calcium ion; response to amine stimulus
Product References and Citations for anti-GluN2A/NR2A antibody
1. Teng H.J., et al. (2010) PLoS ONE. 5: e13342.
Research Articles on GluN2A/NR2A
1. Results revealed a specific regulation by SHP1 of GluN2A-dependent pain transmission. Authors provided evidence that interference with SHP1/GluN2A interaction in spinal dorsal horn generated an effective analgesic action against inflammatory pain.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
