Carbohyde sulfotransferase 2 (CHST2), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CHST2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7200091 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Carbohyde sulfotransferase 2 (CHST2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CHST2. The ELISA analytical biochemical technique of the MBS7200091 kit is based on CHST2 antibody-CHST2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHST2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHST2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Signal Transduction

52,787 Da

carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2

carbohydrate sulfotransferase 2; epididymis secretory protein Li 75; N-acetylglucosamine 6-O-sulfotransferase 1; carbohydrate (chondroitin 6/keratan) sulfotransferase 2; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2

Carbohydrate sulfotransferase 2

GN6ST; GST-2; GlcNAc6ST-1; Gn6ST-1  [Similar Products]

CHST2_HUMAN

This locus encodes a sulfotransferase protein. The encoded enzyme catalyzes the sulfation of a nonreducing N-acetylglucosamine residue, and may play a role in biosynthesis of 6-sulfosialyl Lewis X antigen. [provided by RefSeq, Aug 2011]

CHST2: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Transferase; Glycan Metabolism - keratan sulfate biosynthesis; EC 2.8.2.-; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: Golgi membrane; intrinsic to Golgi membrane; integral to membrane; trans-Golgi network

Molecular Function: sulfotransferase activity; N-acetylglucosamine 6-O-sulfotransferase activity

Biological Process: keratan sulfate metabolic process; sulfur metabolic process; glycosaminoglycan metabolic process; multicellular organismal development; keratan sulfate biosynthetic process; carbohydrate metabolic process; N-acetylglucosamine metabolic process; pathogenesis; inflammatory response

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