Cytokeratin 5, Antibody

K5; DDD; EBS2; KRT5A; KRT5

For Research Use Only. Not for use in diagnostic procedures.

IgG1

3E2F1

Mouse

Ascitic fluid containing 0.03% sodium azide.

Store at 4 degree C, for long term storage, store at-20 degree C

Small volumes of anti-CK5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CK5 (keratin 5) is a member of the keratin gene family. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin5 gene (KRT5) have been shown to result in the autosomal dominant disorderepidermolysis bullosa (EB). Defects in KRT5 are a cause of epidermolysis bullosa simplex.

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Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

WB: 1:500-1:2000
IHC: 1:200-1:1000
ELISA: 1:10000

Figure 1: Western blot analysis using CK5 mouse mAb against truncated CK5 recombinant protein

Figure 2: Immunohistochemical analysis of paraffin-embedded human esophagus epithelium (A), salivary gland basal cell (B), lung squamous cell carcinoma (C), endometrium admosquamous carcinoma (D), showing cytoplasmic and membrane localization using CK5 mouse mAb with DAB staining.

Figure 3: Immunohistochemical analysis of paraffin-embedded human skin tissues using CK5 mouse mAb.

62,378 Da

keratin 5

keratin, type II cytoskeletal 5

Keratin, type II cytoskeletal 5

CK-5; K5  [Similar Products]

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

MiscellaneousThere are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

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