ACE2, Polyclonal Antibody

ACEH; Angiotensin-converting enzyme 2; ACE-related carboxypeptidase; ACEH; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Anti-ACE2 has no cross response to ACE1.

ACE2 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL (lot specific)

ACE2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Small volumes of anti-ACE2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

ACE2 Antibody: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

ACE2 antibody can be used for the detection of ACE2 by Western blot at 0.5 to 2 mug/mL. A 90 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 20 mug/mL.

Western blot analysis of ACE2 in human kidney lysate with ACE2 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.

Immunohistochemical staining of human kidney tissue using ACE2 antibody at 2 μg/mL.

Immunofluorescence of ACE2 in Human Kidney cells with ACE2 antibody at 20 μg/mL.

63,912 Da

angiotensin I converting enzyme 2

angiotensin-converting enzyme 2; peptidyl-dipeptidase A; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin-converting enzyme homolog; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2

Angiotensin-converting enzyme 2

ACEH  [Similar Products]

ACE2_HUMAN

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protease; EC 3.4.17.23

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: extracellular space; cell surface; cytoplasm; extracellular region; plasma membrane; integral to membrane; lipid raft

Molecular Function: peptidyl-dipeptidase activity; viral receptor activity; protein binding; carboxypeptidase activity; metallopeptidase activity; zinc ion binding; peptide hormone binding; endopeptidase activity; glycoprotein binding

Biological Process: virion attachment, binding of host cell surface receptor; entry of virus into host cell; maternal process involved in pregnancy; regulation of vasodilation; angiotensin maturation; proteolysis; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of cytokine production; regulation of cell proliferation; cellular protein metabolic process; regulation of vasoconstriction; regulation of inflammatory response; angiotensin catabolic process in blood; receptor biosynthetic process; angiotensin mediated drinking behavior

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