JAM-C, Monoclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

CRAM-19 H36

Rat

Clone CRAM-19 H36 recognises mouse and human Junctional adhesion molecule C (JAM-C), also known as JAM-3 and, historically, as JAM-2.JAM-C is expressed at junctions between endothelial and epithelial cells, as well as on leukocytes, platelets, vascular smooth muscle cells and fibroblasts, amongst other cell types. It plays a role in tight junctions and inflammatory processes and interacts with JAM-A and JAM-B.

FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

IgG concentration 0.1 mg/ml (lot specific)

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Small volumes of anti-JAM-C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Clone CRAM-19 H36 recognises mouse and human Junctional adhesion molecule C (JAM-C), also known as JAM-3 and, historically, as JAM-2.JAM-C is expressed at junctions between endothelial and epithelial cells, as well as on leukocytes, platelets, vascular smooth muscle cells and fibroblasts, amongst other cell types. It plays a role in tight junctions and inflammatory processes and interacts with JAM-A and JAM-B.

Flow cytometry (FC/FACS), Immunofluorescence (IF)

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Staining of JAM-C transfected CHO cells with Rat anti Mouse JAM-C:FITC (MBS224678)

Staining of JAM-C transfected CHO cells with Rat anti Mouse JAM-C (MBS224732) followed by Rabbit F(ab')2 anti Rat IgG:FITC (MBS235146)

Staining of JAM-C transfected CHO cells with Rat anti Mouse JAM-C:Biotin (MBS224659) followed by Streptavidin:FITC (MBS238412)

34,838 Da

junction adhesion molecule 3

junctional adhesion molecule C; junction cell adhesion molecule 3; junction cell adhesion molecule C; junctional adhesion molecule 3

Junctional adhesion molecule C

JAM-C; JAM-3  [Similar Products]

JAM3_MOUSE

JAM3: Participates in cell-cell adhesion. It is a counter- receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis. Defects in JAM3 are the cause of hemorrhagic destruction of the brain with subependymal calcification and cataracts (HDBSCC). A syndrome characterized by congenital cataracts and severe brain abnormalities apparently resulting from hemorrhagic destruction of the brain tissue, including the cerebral white matter and basal ganglia. Patients manifest profound developmental delay, and other neurologic features included seizures, spasticity, and hyperreflexia. Brain imaging shows multifocal intraparenchymal hemorrhage with associated liquefaction and massive cystic degeneration, and calcification in the subependymal region and in brain tissue. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Cellular Component: Golgi apparatus; extracellular space; desmosome; tight junction; cell surface; membrane; plasma membrane; extracellular region; integral to membrane; intercellular junction; cell junction

Molecular Function: integrin binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: myelination; adaptive immune response; cell migration; cell-matrix adhesion; transmission of nerve impulse; neutrophil homeostasis; leukocyte migration during inflammatory response; myeloid progenitor cell differentiation; establishment of cell polarity; spermatogenesis; angiogenesis; cell adhesion; spermatid development

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