hect domain and RLD 4, ELISA Kit

Mouse Probable E3 ubiquitin-protein ligase HERC4 (HERC4) ELISA kit; DKFZp564G092; KIAA1593; ; hect domain and RLD 4

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of HERC4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9340498 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the hect domain and RLD 4 (HERC4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HERC4. The ELISA analytical biochemical technique of the MBS9340498 kit is based on HERC4 antibody-HERC4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HERC4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HERC4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

118,412 Da

hect domain and RLD 4

probable E3 ubiquitin-protein ligase HERC4; HECT domain and RCC1-like domain-containing protein 4

Probable E3 ubiquitin-protein ligase HERC4

HERC4_MOUSE

HERC4: Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase; Ligase

Cellular Component: cytoplasm; nucleus

Molecular Function: coenzyme F420-2 alpha-glutamyl ligase activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; spermatogenesis; cell differentiation

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