Product Name
Delta-like 1 (DLL3), Monoclonal Antibody
Popular Item
Full Product Name
Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172) (APC)
Product Synonym Names
Anti -Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172) (APC)
Product Gene Name
anti-DLL3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 19; NC_000019.9 (39989557..39999121). Location: 19q13
3D Structure
ModBase 3D Structure for Q9NYJ7
Specificity
Recognizes human Delta-Like 1. Does not crossreact with recombinant mouse DLL4
Purity/Purification
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in saline buffer, 0.5% BSA and 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Concentration
0.01 mg/ml (lot specific)
Immunogen
Recombinant protein corresponding to Ser22-Gly540 of human Delta Like Protein 1 extracelular domain expressed in NSO cells
Flow Cytometry
1. T98G cells. may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of D2727-70C to <=1x10e6 cells and incubated for 30 minutes at RT.
3. Wash the cells twice in MBS635370 Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspended the cells in MBS635370 Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2B antibody. This procedure may need to be modified, depending upon the cell type and final utilization. Individual users may need to titrate to determine the optimal reagent amount for their specific use.
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C. Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Freezing APC conjugates will result in a substantial loss of activity. APC conjugates are sensitive to light.
Other Notes
Small volumes of anti-DLL3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DLL3 antibody
DLL1 is the human homolog of the Drosophila Notch ligand, Delta. It is a type I transmembrane protein with a DSL (Delta, Serrate, Jagged) domain followed by 8 EGF-like domains in its extracellular region and a short cytoplasmic region. Notch-DSL signaling plays an important role in the differentiation processes of many tissues.
Product Categories/Family for anti-DLL3 antibody
Antibodies; Abs to Notch Proteins
Applications Tested/Suitable for anti-DLL3 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-DLL3 antibody
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 10ul labels 1x10e6.
NCBI/Uniprot data below describe general gene information for DLL3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_058637.1
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NCBI GenBank Nucleotide #
NM_016941.3
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UniProt Primary Accession #
Q9NYJ7
[Other Products]
UniProt Secondary Accession #
Q8NBS4; E9PFG2[Other Products]
UniProt Related Accession #
Q9NYJ7[Other Products]
Molecular Weight
64,618 Da[Similar Products]
NCBI Official Full Name
delta-like protein 3 isoform 1
NCBI Official Synonym Full Names
delta-like 3 (Drosophila)
NCBI Official Symbol
DLL3 [Similar Products]
NCBI Official Synonym Symbols
SCDO1
[Similar Products]
NCBI Protein Information
delta-like protein 3; delta3; drosophila Delta homolog 3
UniProt Protein Name
Delta-like protein 3
UniProt Synonym Protein Names
Drosophila Delta homolog 3
Protein Family
Delta-like protein
UniProt Gene Name
DLL3 [Similar Products]
UniProt Synonym Gene Names
Delta3 [Similar Products]
UniProt Entry Name
DLL3_HUMAN
NCBI Summary for DLL3
This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for DLL3
DLL3: Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. Can bind and activate Notch-1 or another Notch receptor.
Protein type: Membrane protein, integral; Cell development/differentiation
Chromosomal Location of Human Ortholog: 19q13
Cellular Component: integral to membrane
Molecular Function: Notch binding
Biological Process: paraxial mesoderm development; compartment specification; somitogenesis; Notch signaling pathway; skeletal development; negative regulation of neurogenesis
Disease: Spondylocostal Dysostosis 1, Autosomal Recessive
Research Articles on DLL3
1. DLL3 was silenced by methylation in human human hepatocellular carcinoma and it negatively regulates the growth of human hepatocellular carcinoma cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
