Delta-like 1, Monoclonal Antibody

Anti -Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 19; NC_000019.9 (39989557..39999121). Location: 19q13

Monoclonal

IgG2b

7H56

Mouse

Recognizes recombinant human DLL1 in direct ELISAs. Does not crossreact with recombinant human DLL3 or recombinant human DLL4.

Affinity Purified
Purified by Protein G affinity chromatography.

Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 200ul sterile PBS.

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-DLL3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Delta-like protein 1 (DLL1) is a 90-100kD type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23aa transmembrane segment and a 155aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37% and 54% aa sequence identity with DLL2, 3 and 4, respectively. A 60kD ECD fragment released by ADAM9, 12 or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma-secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

Antibodies; Abs to Notch Proteins

ELISA (EL/EIA), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Suitable for use in ELISA, Immunocytochemistry and Immunohistochemistry.
Dilution: Immunocytochemistry: 8-25ug/ml detects DLL1 in immersion fixed HepG2 human hepatocellular carcinoma cell line.
Immunohistochemistry: 8-25ug/ml detects DLL1 in immersion fixed paraffin embedded human squamous cell carcinoma tissue sections.
Optimal dilutions should be determined by the researcher.

64,618 Da[Similar Products]

delta-like 3 (Drosophila)

delta-like protein 3; delta3; drosophila Delta homolog 3

Delta-like protein 3

Delta3  [Similar Products]

DLL3_HUMAN

This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

DLL3: Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. Can bind and activate Notch-1 or another Notch receptor.

Protein type: Membrane protein, integral; Cell development/differentiation

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: integral to membrane

Molecular Function: Notch binding

Biological Process: compartment specification; paraxial mesoderm development; Notch signaling pathway; somitogenesis; skeletal development; negative regulation of neurogenesis

Disease: Spondylocostal Dysostosis 1, Autosomal Recessive

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.