Full Product Name
SUR1 Antibody, Clone S289-16: Dylight 405
Product Synonym Names
Mouse Anti-Rat SUR1 Monoclonal IgG1; SUR1 Antibody: Dylight 405; AM60008PU-N Antibody; ABC36 Antibody; Abcc8 Antibody; ATP binding cassette sub family C member 8 Antibody; HHF1 Antibody; HRINS Antibody; MRP8 Antibody; PHHI Antibody; SUR Antibody; SUR1 Antibody; Sulfonylurea receptor (hyperinsulinemia) Antibody; ATP binding cassette sub family C (CFTR/MRP) member 8 Antibody; ATP binding cassette transporter sub family C member 8 (1) Antibody; ATP-binding cassette sub-family C member 8 Antibody; HI Antibody; PHHI Antibody; Sulfonylurea receptor 1 Antibody; SUR1delta2 Antibody; TNDM2 Antibody
Product Gene Name
anti-SUR1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q09429
Species Reactivity
Human, Mouse, Rat, Hamster
Specificity
Detects ~160 kDa. Does not cross-react with SUR2B.
Purity/Purification
Protein G Purified
Form/Format
PBS pH 7.4, 50% Glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Fusion protein amino acids 1548-1582 (cytoplasmic C-terminus) of rat SUR1
Cellular Localization
Membrane
Certificate of Analysis
1 ug/ml was sufficient for detection of SUR1 in 20 ug of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, Förster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-SUR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SUR1 antibody
Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir6.x potassium channel. Hence the KATP channel monitors the energy balance within the cell (2).
Product Categories/Family for anti-SUR1 antibody
Neuroscience; Cell Markers; Neuron Markers; Membrane Markers; Cell Signaling; Cancer; Cardiovascular System; Atherosclerosis; Heart
Applications Tested/Suitable for anti-SUR1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-SUR1 antibody
WB: 1:1000
IHC: 1:1000
ICC/IF: 1:100
Immunocytochemistry (ICC) of anti-SUR1 antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SUR1 Antibody. (D) Composite.
Western Blot (WB) of anti-SUR1 antibody
Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.
NCBI/Uniprot data below describe general gene information for SUR1. It may not necessarily be applicable to this product.
NCBI Accession #
Q09429.4
[Other Products]
UniProt Primary Accession #
Q09429
[Other Products]
UniProt Related Accession #
Q09429[Other Products]
NCBI Official Full Name
ATP-binding cassette sub-family C member 8
NCBI Official Synonym Full Names
ATP binding cassette subfamily C member 8
NCBI Official Symbol
Abcc8 [Similar Products]
NCBI Official Synonym Symbols
Sur; Sur1
[Similar Products]
NCBI Protein Information
ATP-binding cassette sub-family C member 8
UniProt Protein Name
ATP-binding cassette sub-family C member 8
UniProt Synonym Protein Names
Sulfonylurea receptor 1
Protein Family
S-alkyl-thiohydroximate lyase
UniProt Gene Name
Abcc8 [Similar Products]
UniProt Synonym Gene Names
Sur; Sur1 [Similar Products]
UniProt Entry Name
ABCC8_RAT
NCBI Summary for SUR1
binds sulfonylurea; subunit of the ATP-sensitive potassium channel K(ATP), which is composed of Sur1 and the inwardly rectifying K(+) channel subunit, Kir6.2 [RGD, Feb 2006]
UniProt Comments for SUR1
Subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K+ channels and insulin release.
Product References and Citations for anti-SUR1 antibody
1. Campbell J.D., Sansom M.S., Ashcroft F.M. (2003) EMBO Resp. 4(11): 1038-1042. 2. Nichols C.G. (2006) Nature. 440 (7083): 470-476.
Research Articles on SUR1
1. Intricate interactions between TMD0, the loop following TMD0, and Kir6.2 near the proposed PIP2 , and where ATP density is observed, suggest SUR1 may contribute to ATP and PIP2 binding to enhance Kir6.2 sensitivity to both.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
