Full Product Name
Bcr Rabbit pab Antibody
Product Synonym Names
BCR; Bcr ; Bcr ; BCR; ALL; BCR1; CML; D22S11; D22S662; FLJ16453; PHL
Product Gene Name
anti-BCR antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 22; NC_000022.10 (23522402..23660224). Location: 22q11.23
3D Structure
ModBase 3D Structure for P11274
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity purification
Immunogen
A synthetic -peptide of human Bcr
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol,
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Other Notes
Small volumes of anti-BCR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BCR antibody
Rabbit polyclonal to Bcr
The Bcr gene was orginally identified by its presence in the chimeric Bcr-Abl oncogene (1). The amino-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domains. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region may be involved in a GTPase activating function for the small GTP-binding protein Rac (2,3). The function of wild type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstream signaling mediator (4). The Bcr-Abl fusion results in production of a constitutively active tyrosine kinase, which causes chronic myelogenous leukemia (CML) (5). Tyr177 of Bcr is phosphorylated in the Bcr-Abl fusion protein, which plays an important role in transforming the activity of Bcr-Abl (6). Phosphorylated Tyr177 of Bcr provides a docking site for Gab2 and GRB2 (7,8).
Applications Tested/Suitable for anti-BCR antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-BCR antibody
Western Blot (WB): 1/1000-2000
Immunohistochemistry (IHC): 1/50
Immunocytochemistry (ICC): 1/100
Immunoprecipitation (IP): 1/30
Flow Cytometry (FC/FACS): 1/20
Western Blot (WB) of anti-BCR antibody
Western blot analysis of extracts of various cell lines, using BCR antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry (IHC) of anti-BCR antibody
Immunohistochemistry of paraffin-embedded human stomach using BCR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry (IHC) of anti-BCR antibody
Immunohistochemistry of paraffin-embedded human prostate using BCR antibody at dilution of 1:100 (40x lens).
NCBI/Uniprot data below describe general gene information for BCR. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004318.3
[Other Products]
NCBI GenBank Nucleotide #
NM_004327.3
[Other Products]
UniProt Primary Accession #
P11274
[Other Products]
UniProt Secondary Accession #
P78501; Q12842; Q4LE80; Q6NZI3[Other Products]
UniProt Related Accession #
P11274[Other Products]
Molecular Weight
1271[Similar Products]
NCBI Official Full Name
breakpoint cluster region protein isoform 1
NCBI Official Synonym Full Names
breakpoint cluster region
NCBI Official Symbol
BCR [Similar Products]
NCBI Official Synonym Symbols
ALL; CML; PHL; BCR1; D22S11; D22S662
[Similar Products]
NCBI Protein Information
breakpoint cluster region protein; BCR/FGFR1 chimera protein; FGFR1/BCR chimera protein; renal carcinoma antigen NY-REN-26
UniProt Protein Name
Breakpoint cluster region protein
UniProt Synonym Protein Names
Renal carcinoma antigen NY-REN-26
Protein Family
Bicyclomycin resistance protein
UniProt Gene Name
BCR [Similar Products]
UniProt Synonym Gene Names
BCR1; D22S11 [Similar Products]
UniProt Entry Name
BCR_HUMAN
Product References and Citations for anti-BCR antibody
1. Groffen, J. et al. (1984) Cell 36, 93-99. 2. Maru, Y. et al. (1991) Cell 67, 459-468. 3. Che, W. et al. (2001) Circulation 104, 1399-1406. 4. Abe, J. I. et al. (2001) Ann. N.Y. Acad. Sci. 947, 341-343. 5. Voncken, J. W. et al. (1995) Cell 80, 719-728. 6. He, Y. et al. (2002) Blood 99, 2957-2968. 7. Sattler, M. et al. (2002) Cancer Cell 1, 479-492. 8. Warmuth, M. et al. (1995) J. Biol. Chem. 272, 33260-33270.
Precautions
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Disclaimer
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