Bcr, Polyclonal Antibody

Anti -Bcr, phosphorylated (Tyr276) (Breakpoint Cluster Region Protein, Renal Carcinoma Antigen NY-REN-26, BCR1, D22S11)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 22; NC_000022.10 (23522402..23660224). Location: 22q11.23

Polyclonal

IgG

Rabbit

Recognizes human Bcr, phosphorylated at Y276. Species Crossreactivity

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-BCR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Bcr, receptor tyrosine kinase (also known as breakpoint cluster region or C-abl oncogene 1) is an intracellular protein belonging to the protein kinase superfamily and Tyr protein kinase family. It is a proto-oncogene with a SH2 domain, a SH3 domain and a protein kinase domain. Purified Bcr is known to contain autophosphorylation activity and transphosphorylation activity for several protein substrates. The function of this protein includes promoting the exchange of RAC or CDC42-bound GDP by GTP thereby activating them and hence acting as a GTPase activating protein. Moreover, PCAF and BCR signalling are essential for apoptotic cell death (PCAF and BCR signalling control cooperatively pre-mature B cell apoptosis via both depletions of DFFA and BIRC2). Ubiquitous expression of this protein is seen in most tissues. Bcr with Abl1, in reciprocal translocation between chromosomes 22 and 9, produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia.

Antibodies; Abs to Protein Kinases

Western Blot (WB)

Suitable for use in Western Blot.
Dilution: Western Blot: 0.01-1ug/ml

142,819 Da[Similar Products]

breakpoint cluster region

breakpoint cluster region protein; BCR/FGFR1 chimera protein; FGFR1/BCR chimera protein; renal carcinoma antigen NY-REN-26

Breakpoint cluster region protein

BCR1; D22S11  [Similar Products]

BCR_HUMAN

A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Bcr: a protein with serine/threonine protein kinase activity and is a GTPase-activating protein (GAP) for RAC1 and CDC42. The amino-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domains. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. A breakpoint cluster region protein that participates in a t(9;22)(q34;q11) chromosomal translocation that produces a BCR-ABL oncogene responsible for chronic myeloid leukemia (CML), acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The function of wild type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstream signaling mediator. Tyr177 of human Bcr, phosphorylated in the Bcr-Abl fusion protein, provides a docking site for Gab2 and GRB2, and is important in the transforming activity of Bcr-Abl. Sequence variants of the Bcr protein may be associated with bipolar disorder. Two alternatively spliced isoforms of the human protein have been reported.

Protein type: Kinase, protein; EC 2.7.11.1; GEFs, Rac/Rho; Oncoprotein; GAPs, Rac/Rho; Protein kinase, atypical; Protein kinase, Ser/Thr (non-receptor); GAPs; ATYPICAL group; BCR family

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: postsynaptic membrane; protein complex; membrane; postsynaptic density; cytosol; cell junction

Molecular Function: protein serine/threonine kinase activity; Rho guanyl-nucleotide exchange factor activity; protein binding; enzyme binding; protein-tyrosine kinase activity; kinase activity; ATP binding; GTPase activator activity

Biological Process: inner ear morphogenesis; peptidyl-tyrosine phosphorylation; regulation of cell cycle; protein amino acid autophosphorylation; response to lipopolysaccharide; platelet-derived growth factor receptor signaling pathway; signal transduction; protein amino acid phosphorylation; negative regulation of neutrophil degranulation; regulation of small GTPase mediated signal transduction; positive regulation of phagocytosis; small GTPase mediated signal transduction; negative regulation of inflammatory response; brain development; actin cytoskeleton organization and biogenesis; neuromuscular process controlling balance; negative regulation of cell migration

Disease: Leukemia, Acute Lymphoblastic; Leukemia, Chronic Myeloid

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