Product Name
Sprouty-Related, EVH1 Domain Containing 1 (SPRED1), ELISA Kit
Full Product Name
Rat Sprouty-Related, EVH1 Domain Containing 1 ELISA Kit
Product Gene Name
SPRED1 elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
Reproducibility
Intra-Assay: CV<10%
Inter-Assay: CV<15%
Reactivity Note
No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.
Preparation and Storage
Store at 2-8 degree C.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SPRED1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SPRED1 purchase
MBS7255536 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SPRED1. The ELISA analytical biochemical technique of the MBS7255536 kit is based on SPRED1 antibody-SPRED1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPRED1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPRED1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SPRED1 elisa kit
This ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the targets.
Product Categories/Family for SPRED1 elisa kit
Signal Transduction
NCBI/Uniprot data below describe general gene information for SPRED1. It may not necessarily be applicable to this product.
NCBI Accession #
EAW92368.1
[Other Products]
UniProt Secondary Accession #
Q05D53; Q8N256; B2RPJ8[Other Products]
UniProt Related Accession #
Q7Z699[Other Products]
Molecular Weight
50,477 Da
NCBI Official Full Name
sprouty-related, EVH1 domain containing 1
NCBI Official Synonym Full Names
sprouty related EVH1 domain containing 1
NCBI Official Symbol
SPRED1 [Similar Products]
NCBI Official Synonym Symbols
NFLS; hSpred1; spred-1; PPP1R147
[Similar Products]
NCBI Protein Information
sprouty-related, EVH1 domain-containing protein 1
UniProt Protein Name
Sprouty-related, EVH1 domain-containing protein 1
Protein Family
Sprouty-related, EVH1 domain-containing protein
UniProt Gene Name
SPRED1 [Similar Products]
UniProt Synonym Gene Names
Spred-1; hSpred1 [Similar Products]
NCBI Summary for SPRED1
The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq, Jul 2008]
UniProt Comments for SPRED1
Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow ().
Research Articles on SPRED1
1. The EVH1 domain of Spred1 binds to the noncatalytic portion of the GAP-related domain of neurofibromin.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.