Full Product Name
BROX Antibody
Product Synonym Names
BRO1 domain-containing protein BROX; BRO1 domain-and CAAX motif-containing protein; BROX; BROFTI C1orf58
Product Gene Name
anti-BROX antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q5VW32
Purity/Purification
>95%
Protein G Purified
Immunogen
Recombinant Human BRO1 domain-containing protein BROX protein (288-409AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-BROX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-BROX antibody
Cell Biology
Applications Tested/Suitable for anti-BROX antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-BROX antibody
WB: 1:500-1:5000
IHC: 1:200-1:500
IF: 1:50-1:200
Immunohistochemistry (IHC) of anti-BROX antibody
IHC image of CSB-PA730136LA01HU diluted at 1:200 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot (WB) of anti-BROX antibody
Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: BROX antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47, 43 kDa
Observed band size: 47 kDa
Immunofluorescence (IF) of anti-BROX antibody
Immunofluorescence staining of Hela cells with CSB-PA730136LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
NCBI/Uniprot data below describe general gene information for BROX. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001275508.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001288579.1
[Other Products]
UniProt Primary Accession #
Q5VW32
[Other Products]
UniProt Secondary Accession #
Q96MG1; B7Z9G5[Other Products]
UniProt Related Accession #
Q5VW32[Other Products]
Molecular Weight
42,872 Da
NCBI Official Full Name
BRO1 domain-containing protein BROX isoform b
NCBI Official Synonym Full Names
BRO1 domain and CAAX motif containing
NCBI Official Symbol
BROX [Similar Products]
NCBI Official Synonym Symbols
C1orf58
[Similar Products]
NCBI Protein Information
BRO1 domain-containing protein BROX
UniProt Protein Name
BRO1 domain-containing protein BROX
UniProt Synonym Protein Names
BRO1 domain- and CAAX motif-containing protein
Protein Family
BRO1 domain-containing protein
UniProt Gene Name
BROX [Similar Products]
UniProt Synonym Gene Names
BROFTI; C1orf58 [Similar Products]
Research Articles on BROX
1. homologous domain of human Bro1 domain-containing proteins, Alix and Brox, binds CHMP4B but not STAM2, despite their high structural similarity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
