Adipose Triglyceride Lipase, Polyclonal Antibody

Anti -Adipose Triglyceride Lipase (ATGL, Desnutrin, Phospholipase A2 zeta)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 11; NC_000011.9 (818901..825573). Location: 11p15.5

Polyclonal

IgG

Rabbit

Recognizes human ATGL. Species Crossreactivity: mouse and rat.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in TBS, pH 7.4, 0.5mg/ml BSA, 0.02% sodium azide, 50% glycerol.

0.4 mg/ml (lot specific)

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-PNPLA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Triglycerides are the most efficient form of energy storage in mammalian adipose tissue during times of caloric excess. ATGL is one of the key enzymes involved in the mobilization of fatty acids from triglyceride stores in adipose tissue, catalyzing the conversion of triacylglycerols to diacylglycerols. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity, and thus may be a potential drug target for the diabetic pathology. ATGL mRNA is detected in a wide range of tissues including adipose, lung, skeletal muscle, testis, heart, brain, and kidney, with adipose tissue expressing the highest level.

Antibodies; Abs to Enzymes, Phospholipase

Western Blot (WB), Immunohistochemistry (IHC)

Suitable for use in Immunohistochemistry and Western Blot.
Dilution: Immunohistochemistry (paraffin and frozen sections): 4ug/ml.
Western Blot: 1:200. Detects a band at 56kD from tissues and cells such as brown fat, liver, murine macrophages, and HepG2 cells.

55,316 Da[Similar Products]

patatin-like phospholipase domain containing 2

patatin-like phospholipase domain-containing protein 2; TTS2.2; desnutrin; IPLA2-zeta; OTTHUMP00000164432; triglyceride hydrolase; adipose triglyceride lipase; transport-secretion protein 2.2; pigment epithelium-derived factor; calcium-independent phospholipase A2

Patatin-like phospholipase domain-containing protein 2

ATGL  [Similar Products]

PLPL2_HUMAN

This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq]

PNPLA2: the rate-limiting lipolytic enzyme in mammals, flies, and yeast. Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Upregulated by exercise training in human skeletal muscle. Has acylglycerol transacylase activity. May act coordinately with HSL within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. Interacting with ABHD5 stimulates its triglyceride hydrolase activity. Despite a colocalization in lipid droplets, it probably does not interact with perilipin. Transcriptionally regulated by FOXO1A. Defects cause neutral lipid storage disease (NLSD), an autosomal recessive disorder characterized by the excessive accumulation of neutral lipids in multiple tissues. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.3; Membrane protein, integral; Lipase

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; integral to membrane; plasma membrane; lipid particle; cytosol

Molecular Function: triacylglycerol lipase activity

Biological Process: sequestering of lipid; phospholipid metabolic process; triacylglycerol catabolic process; glycerophospholipid biosynthetic process

Disease: Neutral Lipid Storage Disease With Myopathy

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