Full Product Name
CD59 siRNA (Rat)
Product Synonym Names
CD59 glycoprotein; MAC-inhibitory protein; MAC-IP; Membrane attack complex inhibition factor; MACIF; Protectin; CD antigen CD59
Product Gene Name
CD59 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P27274
Specificity
CD59 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CD59 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CD59 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CD59 sirna
siRNA to inhibit CD59 expression using RNA interference
Applications Tested/Suitable for CD59 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CD59. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037057.1
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NCBI GenBank Nucleotide #
NM_012925.1
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UniProt Primary Accession #
P27274
[Other Products]
UniProt Related Accession #
P27274[Other Products]
Molecular Weight
13,790 Da
NCBI Official Full Name
CD59 glycoprotein
NCBI Official Synonym Full Names
CD59 molecule, complement regulatory protein
NCBI Official Symbol
Cd59 [Similar Products]
NCBI Official Synonym Symbols
Cd59a; Cd59b; MACIF; MACIP; MAC-IP
[Similar Products]
NCBI Protein Information
CD59 glycoprotein
UniProt Protein Name
CD59 glycoprotein
UniProt Synonym Protein Names
MAC-inhibitory protein; MAC-IP; Membrane attack complex inhibition factor; MACIF; Protectin; CD_antigen: CD59
Protein Family
CD59 glycoprotein
UniProt Gene Name
Cd59 [Similar Products]
UniProt Synonym Gene Names
MAC-IP; MACIF [Similar Products]
UniProt Entry Name
CD59_RAT
NCBI Summary for CD59
may play a role in regulation of complement; may bind complement proteins C8 and C9 [RGD, Feb 2006]
UniProt Comments for CD59
CD59: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).
Protein type: Membrane protein, GPI anchor
Cellular Component: anchored to external side of plasma membrane; extracellular space; compact myelin; cell surface; focal adhesion; plasma membrane; sarcolemma; vesicle
Molecular Function: complement binding
Biological Process: negative regulation of cytolysis; cell surface receptor linked signal transduction; negative regulation of complement activation; regulation of complement activation; negative regulation of activation of membrane attack complex; positive regulation of T cell proliferation; negative regulation of apoptosis
Research Articles on CD59
1. In rats, CD59 and DAF genes are related to the negative effect of sulfasalazine on sperm maturation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
