Full Product Name
SDHC siRNA (Mouse)
Product Synonym Names
Succinate dehydrogenase cytochrome b560 subunit mitochondrial; Integral membrane protein CII-3; QPs-1; QPs1
Product Gene Name
SDHC sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9CZB0
Specificity
SDHC siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SDHC gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SDHC sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SDHC sirna
siRNA to inhibit SDHC expression using RNA interference
Applications Tested/Suitable for SDHC sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SDHC. It may not necessarily be applicable to this product.
NCBI Accession #
NP_079597.2
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NCBI GenBank Nucleotide #
NM_025321.3
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UniProt Primary Accession #
Q9CZB0
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UniProt Secondary Accession #
Q544Q9; Q99JP2; Q9DCU7[Other Products]
UniProt Related Accession #
Q9CZB0[Other Products]
Molecular Weight
18,382 Da
NCBI Official Full Name
succinate dehydrogenase cytochrome b560 subunit, mitochondrial
NCBI Official Synonym Full Names
succinate dehydrogenase complex, subunit C, integral membrane protein
NCBI Official Symbol
Sdhc [Similar Products]
NCBI Official Synonym Symbols
AI316496; AU019277; 0610010E03Rik
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NCBI Protein Information
succinate dehydrogenase cytochrome b560 subunit, mitochondrial
UniProt Protein Name
Succinate dehydrogenase cytochrome b560 subunit, mitochondrial
UniProt Synonym Protein Names
Integral membrane protein CII-3; QPs-1; QPs1
Protein Family
Succinate dehydrogenase 2 membrane
UniProt Gene Name
Sdhc [Similar Products]
UniProt Synonym Gene Names
QPs1 [Similar Products]
UniProt Entry Name
C560_MOUSE
UniProt Comments for SDHC
SDHC: Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHC are the cause of paragangliomas type 3 (PGL3). A neural crest tumor usually derived from the chromoreceptor tissue of a paraganglion. Paragangliomas are most commonly located in the head and neck region, specifically at the carotid bifurcation, the jugular foramen, the vagal nerve, and in the middle ear. Defects in SDHC are a cause of paraganglioma and gastric stromal sarcoma (PGGSS); also known as Carney- Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance. Belongs to the cytochrome b560 family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation; Membrane protein, multi-pass; Carbohydrate Metabolism - citrate (TCA) cycle
Cellular Component: mitochondrion; membrane; mitochondrial inner membrane; integral to membrane
Molecular Function: electron carrier activity; metal ion binding; oxidoreductase activity, acting on the CH-CH group of donors; succinate dehydrogenase activity
Biological Process: tricarboxylic acid cycle; transport
Research Articles on SDHC
1. Studies indicate that the Tet-mev-1 conditional transgenic system can induce mutated SDHC gene in complex II.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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