Steryl-sulfatase, ELISA Kit

STS/Steryl-sulfate sulfohydrolase/Arylsulfatase C (ASC)/Steroid sulfatase/ARSC1

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of STS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS763251 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Steryl-sulfatase (STS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing STS. The ELISA analytical biochemical technique of the MBS763251 kit is based on STS antibody-STS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect STS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, STS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human STS. During the reaction,Human STS in the sample or standard competes with a fixed amount of biotin-labeled Human STS for sites on a pre-coated Monoclonal antibody specific to Human STS. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human STS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

65,492 Da

steroid sulfatase (microsomal), isozyme S

steryl-sulfatase

Steryl-sulfatase

ARSC1; ASC  [Similar Products]

STS_HUMAN

The protein encoded by this gene catalyzes the conversion of sulfated steroid precursors to estrogens during pregnancy. The encoded protein is found in the endoplasmic reticulum, where it acts as a homodimer. Mutations in this gene are known to cause X-linked ichthyosis (XLI). [provided by RefSeq, Jul 2008]

STS: Conversion of sulfated steroid precursors to estrogens during pregnancy. Defects in STS are the cause of ichthyosis X-linked (IXL). Ichthyosis X-linked is a keratinization disorder manifesting with mild erythroderma and generalized exfoliation of the skin within a few weeks after birth. Affected boys later develop large, polygonal, dark brown scales, especially on the neck, extremities, trunk, and buttocks. Belongs to the sulfatase family.

Protein type: EC 3.1.6.2; Hydrolase; Lipid Metabolism - androgen and estrogen; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xp22.32

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum membrane; endosome; Golgi apparatus; intracellular membrane-bound organelle; lysosome; membrane; plasma membrane

Molecular Function: steryl-sulfatase activity; sulfuric ester hydrolase activity

Biological Process: epidermis development; glycosphingolipid metabolic process; post-translational protein modification; steroid catabolic process

Disease: Ichthyosis, X-linked

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