Full Product Name
RP2 siRNA (Mouse)
Product Synonym Names
RP2H; Protein XRP2
Product Gene Name
RP2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9EPK2
Specificity
RP2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RP2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RP2 sirna
siRNA to inhibit RP2 expression using RNA interference
Applications Tested/Suitable for RP2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RP2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001277572.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001290643.1
[Other Products]
UniProt Primary Accession #
Q9EPK2
[Other Products]
UniProt Secondary Accession #
Q8BLN8; Q8BVQ8; Q8BZP9[Other Products]
UniProt Related Accession #
Q9EPK2[Other Products]
Molecular Weight
34,899 Da
NCBI Official Full Name
protein XRP2 isoform a
NCBI Official Synonym Full Names
retinitis pigmentosa 2 homolog (human)
NCBI Official Symbol
Rp2 [Similar Products]
NCBI Official Synonym Symbols
Rp2h; AI662636
[Similar Products]
NCBI Protein Information
protein XRP2
UniProt Protein Name
Protein XRP2
UniProt Gene Name
Rp2 [Similar Products]
UniProt Synonym Gene Names
Rp2h [Similar Products]
UniProt Entry Name
XRP2_MOUSE
UniProt Comments for RP2
RP2: Acts as a GTPase-activating protein (GAP) involved in trafficking between the Golgi and the ciliary membrane. Involved in localization of proteins, such as NPHP3, to the cilium membrane by inducing hydrolysis of GTP ARL3, leading to the release of UNC119 (or UNC119B). Acts as a GTPase-activating protein (GAP) for tubulin in concert with tubulin-specific chaperone C, but does not enhance tubulin heterodimerization. Acts as guanine nucleotide dissociation inhibitor towards ADP-ribosylation factor-like proteins. Defects in RP2 are the cause of retinitis pigmentosa type 2 (RP2); also known as X-linked retinitis pigmentosa 2 (XLRP-2). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the TBCC family.
Protein type: Chaperone
Cellular Component: centriole; Golgi apparatus; cell projection; membrane; cytoplasm; plasma membrane; cytoplasmic vesicle; cilium
Molecular Function: GTP binding; nucleotide binding; nucleoside diphosphate kinase activity; actin binding; GTPase activator activity; ATP binding
Biological Process: GTP biosynthetic process; protein transport; CTP biosynthetic process; transport; UTP biosynthetic process; cell morphogenesis; nucleoside diphosphate phosphorylation; cytoskeleton organization and biogenesis; post-Golgi vesicle-mediated transport; positive regulation of GTPase activity
Research Articles on RP2
1. Our studies suggest that RP2 contributes to the maintenance of photoreceptor function and that cone opsin mislocalization represents an early step in XLRP caused by RP2 mutations.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
