Product Name
FUCA1, Recombinant Protein
Full Product Name
FUCA1, 28-466aa, Human, His tag, Baculovirus
Product Synonym Names
Tissue alpha-L-fucosidase
Product Gene Name
FUCA1 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
VRRAQPPRRY TPDWPSLDSR PLPAWFDEAK FGVFIHWGVF SVPAWGSEWF WWHWQGEGRP QYQRFMRDNY PPGFSYADFG PQFTARFFHP EEWADLFQAA GAKYVVLTTK HHEGFTNWPS PVSWNWNSKD VGPHRDLVGE LGTALRKRNI RYGLYHSLLE WFHPLYLLDK KNGFKTQHFV SAKTMPELYD LVNSYKPDLI WSDGEWECPD TYWNSTNFLS WLYNDSPVKD EVVVNDRWGQ NCSCHHGGYY NCEDKFKPQS LPDHKWEMCT SIDKFSWGYR RDMALSDVTE ESEIISELVQ TVSLGGNYLL NIGPTKDGLI VPIFQERLLA VGKWLSINGE AIYASKPWRV QWEKNTTSVW YTSKGSAVYA IFLHWPENGV LNLESPITTS TTKITMLGIQ GDLKWSTDPD KGLFISLPQL PPSAVPAEFA WTIKLTGVKH HHHHH
3D Structure
ModBase 3D Structure for P04066
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (lot specific)
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of FUCA1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FUCA1 recombinant protein
FUCA1, also known as tissue alpha-L-fucosidase, is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. Fucosidosis is an autosomal recessive lysosomal storage disease resulting from absence of alpha-L-fucosidase activity. Recombinant human FUCA1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for FUCA1 recombinant protein
Signal Transduction; Metabolism
Applications Tested/Suitable for FUCA1 recombinant protein
SDS-PAGE
SDS-Page of FUCA1 recombinant protein
NCBI/Uniprot data below describe general gene information for FUCA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000138
[Other Products]
NCBI GenBank Nucleotide #
NM_000147.4
[Other Products]
UniProt Primary Accession #
P04066
[Other Products]
UniProt Secondary Accession #
Q14334; Q14335; Q3LID0; Q8NAC2; B2RBG3[Other Products]
UniProt Related Accession #
P04066[Other Products]
Molecular Weight
51.7kDa (445aa) 50-70KDa (SDS-PAGE under reducing conditions.)
NCBI Official Full Name
tissue alpha-L-fucosidase
NCBI Official Synonym Full Names
alpha-L-fucosidase 1
NCBI Official Symbol
FUCA1 [Similar Products]
NCBI Official Synonym Symbols
FUCA
[Similar Products]
NCBI Protein Information
tissue alpha-L-fucosidase
UniProt Protein Name
Tissue alpha-L-fucosidase
UniProt Synonym Protein Names
Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; Alpha-L-fucosidase 1
Protein Family
Tissue alpha-L-fucosidase
UniProt Gene Name
FUCA1 [Similar Products]
UniProt Synonym Gene Names
Alpha-L-fucosidase 1 [Similar Products]
NCBI Summary for FUCA1
The protein encoded by this gene is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.[provided by RefSeq, Oct 2009]
UniProt Comments for FUCA1
Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.
Product References and Citations for FUCA1 recombinant protein
Yang M. et al., (1993) Biochim. Biophys. Acta. 1182 (3):245-249.; Seo H.-C. et al., (1994) Hum. Mol. Genet. 3 (11):2065-2066.
Research Articles on FUCA1
1. RNAi-mediated knockdown of endogenous FUCA1 significantly attenuates p53-dependent, chemotherapy-induced apoptotic death.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
