Cytoplasmic dynein 2 heavy chain 1 (DYNC2H1), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of DYNC2H1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7207894 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytoplasmic dynein 2 heavy chain 1 (DYNC2H1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DYNC2H1. The ELISA analytical biochemical technique of the MBS7207894 kit is based on DYNC2H1 antibody-DYNC2H1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DYNC2H1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DYNC2H1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Neurobiology

105,369 Da

dynein, cytoplasmic 2, heavy chain 1

cytoplasmic dynein 2 heavy chain 1; dynein heavy chain 11; dynein heavy chain isotype 1B; dynein heavy chain, isotype 1B; dynein cytoplasmic heavy chain 2; dynein, cytoplasmic, heavy polypeptide 2

Cytoplasmic dynein 2 heavy chain 1

DHC1B; DHC2; DNCH2; DYH1B; KIAA1997; hDHC11  [Similar Products]

DYHC2_HUMAN

This gene encodes a large cytoplasmic dynein protein that is involved in retrograde transport in the cilium and has a role in intraflagellar transport, a process required for ciliary/flagellar assembly. Mutations in this gene cause a heterogeneous spectrum of conditions related to altered primary cilium function and often involve polydactyly, abnormal skeletogenesis, and polycystic kidneys. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jan 2010]

DYNC2H1: May function as a motor for intraflagellar retrograde transport. Functions in cilia biogenesis. May play a role in transport between endoplasmic reticulum and Golgi or organization of the Golgi in cells. Defects in DYNC2H1 are the cause of asphyxiating thoracic dystrophy type 3 (ATD3). ATD3 is an autosomal recessive osteochondrodysplasia which often leads to death in infancy because of a severely constricted thoracic cage and respiratory insufficiency. Defects in DYNC2H1 are the cause of short rib-polydactyly syndrome type 3 (SRPS3); also called Verma-Naumoff syndrome. A lethal skeletal dysplasia characterized by markedly short ribs, short limbs, polydactyly, narrow thorax, and multiple anomalies of major organs, including heart, intestines, genitalia, kidney, liver, and pancreas. Belongs to the dynein heavy chain family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q21-q22.1

Cellular Component: dynein complex; Golgi apparatus; microtubule; apical part of cell; plasma membrane; motile primary cilium; axoneme; cytosol

Molecular Function: ATPase activity; motor activity; microtubule motor activity; ATP binding

Biological Process: asymmetric protein localization; dorsal/ventral pattern formation; organelle organization and biogenesis; forebrain development; antigen processing and presentation of exogenous peptide antigen via MHC class II; positive regulation of smoothened signaling pathway; cilium biogenesis; protein processing; spinal cord motor neuron differentiation; determination of left/right symmetry; Golgi organization and biogenesis; embryonic limb morphogenesis

Disease: Short-rib Thoracic Dysplasia 3 With Or Without Polydactyly

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