Acrosin (ACR), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of ACR. No significant cross-reactivity or interference between ACR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACR and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ACR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7208877 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acrosin (ACR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACR. The ELISA analytical biochemical technique of the MBS7208877 kit is based on ACR antibody-ACR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This ACR ELIACR kit is a 1.5 hour solid-phase ELIACR designed for the quantitative determination of Human ACR. This ELIACR kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||ACR ELIACR kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ACR antibody and an ACR-HRP conjugate. The assay sample and buffer are incubated together with ACR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACR concentration since ACR from samples and ACR-HRP conjugate compete for the anti-ACR antibody . Since the number of sites is limited, as more sites are occupied by ACR from the sample, fewer sites are left to bind ACR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACR concentration in each sample is interpolated from this standard curve.

Cell Biology

45,847 Da

acrosin

acrosin; proacrosin; preproacrosin; acrosin light and heavy chain prepropeptide

Acrosin

ACRS  [Similar Products]

ACRO_HUMAN

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008]

ACR: Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.10; Protease

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: protein complex; extracellular region; Golgi-associated vesicle; acrosomal matrix; nucleus

Molecular Function: mannose binding; protein binding; copper ion binding; amidase activity; DNA binding; zinc ion binding; serine-type endopeptidase activity; drug binding; fucose binding

Biological Process: response to steroid hormone stimulus; binding of sperm to zona pellucida; single fertilization; acrosome reaction; adenylate cyclase activation; penetration of zona pellucida; multicellular organism reproduction; acrosome matrix dispersal

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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