aldehyde dehydrogenase 5 family, member A1, ELISA Kit

Human Succinate-semialdehyde dehydrogenase; mitochondrial (ALDH5A1) ELISA kit; SSADH; SSDH; NAD (+) -dependent succinic semialdehyde dehydrogenase; aldehyde dehydrogenase 5A1; mitochondrial succinate semialdehyde dehydrogenase; succinate-semialdehyde dehydrogenase; aldehyde dehydrogenase 5 family; member A1

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of human ALDH5A1. No significant cross-reactivity or interference between human ALDH5A1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of ALDH5A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS915233 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the aldehyde dehydrogenase 5 family, member A1 (ALDH5A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ALDH5A1. The ELISA analytical biochemical technique of the MBS915233 kit is based on ALDH5A1 antibody-ALDH5A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALDH5A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALDH5A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ALDH5A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALDH5A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALDH5A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALDH5A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

57,215 Da

aldehyde dehydrogenase 5 family, member A1

succinate-semialdehyde dehydrogenase, mitochondrial; aldehyde dehydrogenase 5 family member A1; mitochondrial succinate semialdehyde dehydrogenase; NAD(+)-dependent succinic semialdehyde dehydrogenase

Succinate-semialdehyde dehydrogenase, mitochondrial

SSADH  [Similar Products]

SSDH_HUMAN

This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

ALDH5A1: Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). Defects in ALDH5A1 are the cause of succinate semialdehyde dehydrogenase deficiency (SSADH deficiency). SSADH deficiency is a rare inborn error in the metabolism of 4-aminobutyric acid (GABA) which leads to accumulation of 4-hydroxybutyric acid in physiologic fluids of patients. The disease is characterized by severe ataxia and by mildly retarded psychomotor development. Belongs to the aldehyde dehydrogenase family.

Protein type: Carbohydrate Metabolism - butanoate; Mitochondrial; EC 1.2.1.24; Oxidoreductase; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 6p22

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: aldehyde dehydrogenase (NAD) activity; protein homodimerization activity; succinate-semialdehyde dehydrogenase activity; succinate-semialdehyde dehydrogenase [NAD(P)+] activity

Biological Process: succinate metabolic process; glutamate metabolic process; central nervous system development; neurotransmitter catabolic process; short-chain fatty acid metabolic process; neurotransmitter secretion; acetate metabolic process; glucose metabolic process; glutamine metabolic process; glucosylceramide metabolic process; post-embryonic development; protein homotetramerization; synaptic transmission; glutathione metabolic process; galactosylceramide metabolic process; glycerophospholipid metabolic process; gamma-aminobutyric acid catabolic process

Disease: Succinic Semialdehyde Dehydrogenase Deficiency

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