Product Name
FGFRL1, siRNA
Full Product Name
FGFRL1 siRNA (Human)
Product Synonym Names
FGFR5; FHFR; Fibroblast growth factor receptor-like 1; FGF receptor-like protein 1; FGF homologous factor receptor; FGFR-like protein; Fibroblast growth factor receptor 5; FGFR-5
Product Gene Name
FGFRL1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8N441
Specificity
FGFRL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FGFRL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of FGFRL1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FGFRL1 sirna
siRNA to inhibit FGFRL1 expression using RNA interference
Applications Tested/Suitable for FGFRL1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for FGFRL1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001004356.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001004356.2
[Other Products]
UniProt Primary Accession #
Q8N441
[Other Products]
UniProt Secondary Accession #
Q6PJN1; Q9BXN7; Q9H4D7; B2RAU9[Other Products]
UniProt Related Accession #
Q8N441[Other Products]
Molecular Weight
54,537 Da
NCBI Official Full Name
fibroblast growth factor receptor-like 1
NCBI Official Synonym Full Names
fibroblast growth factor receptor-like 1
NCBI Official Symbol
FGFRL1 [Similar Products]
NCBI Official Synonym Symbols
FHFR; FGFR5
[Similar Products]
NCBI Protein Information
fibroblast growth factor receptor-like 1
UniProt Protein Name
Fibroblast growth factor receptor-like 1
UniProt Synonym Protein Names
FGF homologous factor receptor; FGFR-like protein; Fibroblast growth factor receptor 5; FGFR-5
Protein Family
Fibroblast growth factor receptor
UniProt Gene Name
FGFRL1 [Similar Products]
UniProt Synonym Gene Names
FGFR5; FHFR; FGF receptor-like protein 1; FGFR-5 [Similar Products]
UniProt Entry Name
FGRL1_HUMAN
NCBI Summary for FGFRL1
The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A marked difference between this gene product and the other family members is its lack of a cytoplasmic tyrosine kinase domain. The result is a transmembrane receptor that could interact with other family members and potentially inhibit signaling. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for FGFRL1
FGFRL1: Has a negative effect on cell proliferation.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 4p16
Cellular Component: Golgi apparatus; transport vesicle; plasma membrane; integral to membrane
Molecular Function: heparin binding; fibroblast growth factor binding; fibroblast growth factor receptor activity
Biological Process: negative regulation of cell proliferation; fibroblast growth factor receptor signaling pathway; protein heterooligomerization; skeletal development; protein homooligomerization
Disease: Wolf-hirschhorn Syndrome
Research Articles on FGFRL1
1. The signaling complex appears to integrate the input from FGFR and EphA4, and release the output signal through FRS2alpha.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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