Full Product Name
AF10 Antibody
Product Synonym Names
Protein AF-10; MLLT10; AF10
Product Gene Name
anti-AF10 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P55197
Species Reactivity
Human, Mouse
Specificity
The antibody detects endogenous levels of total AF10 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Immunogen Description
Synthesized peptide derived from internal of human AF10.
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-AF10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AF10 antibody
Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA.Chaplin T., Blood 85:1435-1441(1995).Deloukas P., Nature 429:375-381(2004).Matsuoka S., Science 316:1160-1166(2007).
Product Categories/Family for anti-AF10 antibody
Total protein Ab
Applications Tested/Suitable for anti-AF10 antibody
Western Blot (WB)
Application Notes for anti-AF10 antibody
Western blotting: 1:500~1:3000
Western Blot (WB) of anti-AF10 antibody
Western blot analysis of extracts from COLO205 cells and Jurkat cells, using AF10 antibody.
NCBI/Uniprot data below describe general gene information for AF10. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001182555.1
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NCBI GenBank Nucleotide #
NM_001195626.1
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UniProt Primary Accession #
P55197
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UniProt Secondary Accession #
Q5JT37; Q5VX90; Q66K63; B1ANA8[Other Products]
UniProt Related Accession #
P55197[Other Products]
Molecular Weight
20,047 Da
NCBI Official Full Name
protein AF-10 isoform c
NCBI Official Synonym Full Names
myeloid/lymphoid or mixed-lineage leukemia; translocated to, 10
NCBI Official Symbol
MLLT10 [Similar Products]
NCBI Official Synonym Symbols
AF10
[Similar Products]
NCBI Protein Information
protein AF-10
UniProt Protein Name
Protein AF-10
UniProt Synonym Protein Names
ALL1-fused gene from chromosome 10 protein
UniProt Gene Name
MLLT10 [Similar Products]
UniProt Synonym Gene Names
AF10 [Similar Products]
UniProt Entry Name
AF10_HUMAN
NCBI Summary for AF10
This gene encodes a transcription factor and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]
UniProt Comments for AF10
MLLT10: Probably involved in transcriptional regulation. In vitro or as fusion protein with MLL has transactivation activity. Binds to cruciform DNA. A chromosomal aberration involving MLLT10 is associated with acute leukemias. Translocation t(10;11)(p12;q23) with MLL/HRX. The result is a rogue activator protein. A chromosomal aberration involving MLLT10 is associated with diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with PICALM. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; Oncoprotein
Chromosomal Location of Human Ortholog: 10p12
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: protein binding; DNA binding; zinc ion binding; transcription factor activity
Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter
Disease: Leukemia, Acute Myeloid
Research Articles on AF10
1. we report that variants at the MLLT10 locus are unlikely to alter risk of glioma, and they have no prognostic value among patients with high-grade tumors (glioblastoma).
Precautions
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Disclaimer
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