B-Cell Leukemia/Lymphoma 2 (Bcl2), Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against Bcl2. It has been selected for its ability to recognize Bcl2 in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Small volumes of anti-Bcl2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Knock-Out Validation Antibody

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human U937 Cells; Lane3: Mouse Pancreas Tissue.

Western Blot: Sample: Recombinant Bcl2, Human.

Knockout Validation: Lane 1: Wild-type Jurkat cell lysate;;Lane 2: Bcl2 knockout Jurkat cell lysate;;Predicted MW: 26kDa ;Observed MW: 28kDa;Primary Ab: 3ug/ml Rabbit Anti-Human Bcl2 Ab;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047)

22,337 Da

BCL2, apoptosis regulator

apoptosis regulator Bcl-2

Apoptosis regulator Bcl-2

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Bcl-2: a antiapoptotic member of the Bcl-2 family. Regulates cell death by controlling the mitochondrial membrane permeability. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Phosphorylation by JNKs may increase its antiapoptotic functions.

Protein type: Apoptosis; Autophagy; Membrane protein, integral; Oncoprotein

Chromosomal Location of Human Ortholog: 18q21.33

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; endoplasmic reticulum membrane; membrane; mitochondrial outer membrane; mitochondrion; myelin sheath; nuclear membrane; nucleoplasm; nucleus; pore complex; protein complex

Molecular Function: BH3 domain binding; channel activity; channel inhibitor activity; identical protein binding; protease binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein phosphatase 2A binding; sequence-specific DNA binding; transcription factor binding; ubiquitin protein ligase binding

Biological Process: actin filament organization; apoptosis; axon regeneration; axonogenesis; B cell homeostasis; B cell lineage commitment; B cell proliferation; B cell receptor signaling pathway; behavioral fear response; branching involved in ureteric bud morphogenesis; CD8-positive, alpha-beta T cell lineage commitment; cell aging; cell growth; cellular response to DNA damage stimulus; cellular response to glucose starvation; cellular response to hypoxia; cellular response to organic substance; cochlear nucleus development; defense response to virus; digestive tract morphogenesis; DNA damage response, signal transduction resulting in induction of apoptosis; ear development; endoplasmic reticulum calcium ion homeostasis; female pregnancy; focal adhesion assembly; gland morphogenesis; glomerulus development; hair follicle morphogenesis; homeostasis of number of cells within a tissue; humoral immune response; induction of apoptosis by oxidative stress; induction of apoptosis via death domain receptors; lymphoid progenitor cell differentiation; male gonad development; melanin metabolic process; melanocyte differentiation; mesenchymal cell development; metanephros development; negative regulation of anoikis; negative regulation of apoptosis; negative regulation of apoptotic signaling pathway; negative regulation of autophagy; negative regulation of calcium ion transport into cytosol; negative regulation of cell growth; negative regulation of cell migration; negative regulation of cellular pH reduction; negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; negative regulation of G1/S transition of mitotic cell cycle; negative regulation of intrinsic apoptotic signaling pathway; negative regulation of mitochondrial depolarization; negative regulation of myeloid cell apoptosis; negative regulation of neuron apoptosis; negative regulation of ossification; negative regulation of osteoblast proliferation; negative regulation of reactive oxygen species metabolic process; negative regulation of retinal cell programmed cell death; neuron apoptosis; oocyte development; organ growth; ossification; ovarian follicle development; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; pigment granule organization; positive regulation of B cell proliferation; positive regulation of catalytic activity; positive regulation of cell growth; positive regulation of intrinsic apoptotic signaling pathway; positive regulation of melanocyte differentiation; positive regulation of multicellular organism growth; positive regulation of neuron maturation; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; positive regulation of skeletal muscle fiber development; positive regulation of smooth muscle cell migration; post-embryonic development; protein amino acid dephosphorylation; protein polyubiquitination; reactive oxygen species metabolic process; regulation of calcium ion transport; regulation of cell-matrix adhesion; regulation of gene expression; regulation of glycoprotein biosynthetic process; regulation of mitochondrial membrane permeability; regulation of mitochondrial membrane potential; regulation of nitrogen utilization; regulation of protein heterodimerization activity; regulation of protein homodimerization activity; regulation of protein stability; regulation of transmembrane transporter activity; regulation of viral genome replication; release of cytochrome c from mitochondria; renal system process; response to cytokine; response to drug; response to gamma radiation; response to glucocorticoid stimulus; response to hydrogen peroxide; response to iron ion; response to nicotine; response to radiation; response to toxin; response to UV-B; spleen development; T cell differentiation in thymus; T cell homeostasis; thymus development

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