Product Name
ALDH5A1, siRNA
Full Product Name
ALDH5A1 siRNA (Mouse)
Product Synonym Names
Succinate-semialdehyde dehydrogenase mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase
Product Gene Name
ALDH5A1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8BWF0
Specificity
ALDH5A1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ALDH5A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ALDH5A1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ALDH5A1 sirna
siRNA to inhibit ALDH5A1 expression using RNA interference
Applications Tested/Suitable for ALDH5A1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ALDH5A1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_766120.1
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NCBI GenBank Nucleotide #
NM_172532.3
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UniProt Primary Accession #
Q8BWF0
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UniProt Secondary Accession #
Q5SZW1[Other Products]
UniProt Related Accession #
Q8BWF0[Other Products]
Molecular Weight
55,968 Da
NCBI Official Full Name
succinate-semialdehyde dehydrogenase, mitochondrial
NCBI Official Synonym Full Names
aldhehyde dehydrogenase family 5, subfamily A1
NCBI Official Symbol
Aldh5a1 [Similar Products]
NCBI Official Synonym Symbols
Ahd1; Ahd-1; SSADH; Ssdh1; 6330403E24Rik; D630032B01Rik; OTTMUSG00000000613
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NCBI Protein Information
succinate-semialdehyde dehydrogenase, mitochondrial
UniProt Protein Name
Succinate-semialdehyde dehydrogenase, mitochondrial
UniProt Synonym Protein Names
Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase
UniProt Gene Name
Aldh5a1 [Similar Products]
UniProt Entry Name
SSDH_MOUSE
UniProt Comments for ALDH5A1
ALDH5A1: Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). Defects in ALDH5A1 are the cause of succinate semialdehyde dehydrogenase deficiency (SSADH deficiency). SSADH deficiency is a rare inborn error in the metabolism of 4-aminobutyric acid (GABA) which leads to accumulation of 4-hydroxybutyric acid in physiologic fluids of patients. The disease is characterized by severe ataxia and by mildly retarded psychomotor development. Belongs to the aldehyde dehydrogenase family.
Protein type: EC 1.2.1.24; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Carbohydrate Metabolism - butanoate; Oxidoreductase
Cellular Component: mitochondrion
Molecular Function: aldehyde dehydrogenase (NAD) activity; carboxylic acid binding; oxidoreductase activity; succinate-semialdehyde dehydrogenase activity; NAD binding; oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor; succinate-semialdehyde dehydrogenase [NAD(P)+] activity
Biological Process: succinate metabolic process; glutamate metabolic process; neurotransmitter catabolic process; central nervous system development; short-chain fatty acid metabolic process; metabolic process; gamma-aminobutyric acid metabolic process; acetate metabolic process; glucose metabolic process; glucosylceramide metabolic process; glutamine metabolic process; post-embryonic development; protein homotetramerization; glutathione metabolic process; galactosylceramide metabolic process; glycerophospholipid metabolic process; gamma-aminobutyric acid catabolic process
Research Articles on ALDH5A1
1. These data show that enhanced, rather than compromised, GABA(A) receptor-mediated inhibition occurs in cortico-thalamic networks of SSADH(-/-) mice.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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