Dopamine Receptor D4, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of DRD4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS752718 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dopamine Receptor D4 (DRD4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DRD4. The ELISA analytical biochemical technique of the MBS752718 kit is based on DRD4 antibody-DRD4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DRD4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DRD4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: DRD4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DRD4 antibody and an DRD4-HRP conjugate. The assay sample and buffer are incubated together with DRD4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DRD4 concentration since DRD4 from samples and DRD4-HRP conjugate compete for the anti-DRD4 antibody . Since the number of sites is limited, as more sites are occupied by DRD4 from the sample, fewer sites are left to bind DRD4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DRD4 concentration in each sample is interpolated from this standard curve.

Neurobiology

48,361 Da

dopamine receptor D4

D(4) dopamine receptor; D(4) dopamine receptor; dopamine D4 receptor; D(2C) dopamine receptor; seven transmembrane helix receptor

D(4) dopamine receptor

DRD4_HUMAN

This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats; the sequence shown contains four repeats. [provided by RefSeq, Jul 2008]

DRD4: Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Its activity is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cell soma; membrane; integral to plasma membrane; dendritic spine; plasma membrane; terminal button; vesicle membrane; cell cortex

Molecular Function: dopamine D2 receptor-like receptor activity; identical protein binding; protein binding; potassium channel regulator activity; dopamine receptor activity; dopamine binding; drug binding; SH3 domain binding

Biological Process: circadian rhythm; fear response; synaptic transmission, dopaminergic; behavioral fear response; positive regulation of dopamine uptake; ***** locomotory behavior; activation of MAPK activity; short-term memory; regulation of neurotransmitter secretion; positive regulation of kinase activity; regulation of calcium-mediated signaling; behavioral response to ethanol; dopamine metabolic process; dopamine receptor, adenylate cyclase inhibiting pathway; regulation of inhibitory postsynaptic membrane potential; negative regulation of cAMP biosynthetic process; behavioral response to cocaine; negative regulation of protein secretion; negative regulation of adenylate cyclase activity; regulation of dopamine metabolic process; response to amphetamine; social behavior; regulation of circadian rhythm; arachidonic acid secretion; cellular calcium ion homeostasis; olfactory learning; retina development in camera-type eye; response to steroid hormone stimulus; photoperiodism; dopamine receptor signaling pathway; positive regulation of sodium:hydrogen antiporter activity

Disease: Novelty Seeking Personality Trait; Attention Deficit-hyperactivity Disorder

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