Product Name
COLLAGEN I (COL1A2), Polyclonal Antibody
Full Product Name
RABBIT ANTI HUMAN COLLAGEN I
Product Gene Name
anti-COL1A2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 7; NC_000007.13 (94023873..94060544). Location: 7q22.1
3D Structure
ModBase 3D Structure for P08123
Specificity
COLLAGEN I
This item is specific for human Collagen I, reacting with both native and heat denatured proteins, and has been cross-absorbed against Collagen types III, IV and V.
Form/Format
Purified (Purified IgG - lyophilised)
Immunogen
Collagen type I from human placenta
Preparation
Purified IgG prepared by affinity chromatography on immobilized Collagen type I
Preservative Stabilizers
1% Dextran
1% Mannitol
Reconstitution
Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 1% BSA and 0.09% sodium azide is recommended.
Buffer Solution
Phosphate buffered saline
Preparation and Storage
Prior to reconstitution store at +4 degree C.
After reconstitution store at -20 degree C.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life: 12 months from date of reconstitution.
Other Notes
Small volumes of anti-COL1A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-COL1A2 antibody
Immunohistology - Frozen (IHC), ELISA (EIA), Immunohistology - Paraffin (IHC)
Application Notes for anti-COL1A2 antibody
Immunohistology - Frozen: Min Dilution: 1/10
Max Dilution: 1/50
Immunohistology - Paraffin: Min Dilution: 1/10
Max Dilution: 1/50
Application Note: This product requires antigen retrieval using 1mg/ml pepsin in 0.5M acetic acid for 2 hours at 37 degree C and multiple buffer washes prior to staining of paraffin sections.
ELISA: Min Dilution: 1/500
Max Dilution: 1/3000
NCBI/Uniprot data below describe general gene information for COL1A2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000080.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000089.3
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UniProt Primary Accession #
P08123
[Other Products]
UniProt Secondary Accession #
P02464; Q13897; Q13997; Q13998; Q14038; Q14057; Q15177; Q15947; Q16480; Q16511; Q7Z5S6[Other Products]
UniProt Related Accession #
P08123[Other Products]
Molecular Weight
129,314 Da[Similar Products]
NCBI Official Full Name
collagen alpha-2(I) chain
NCBI Official Synonym Full Names
collagen, type I, alpha 2
NCBI Official Symbol
COL1A2 [Similar Products]
NCBI Official Synonym Symbols
OI4
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NCBI Protein Information
collagen alpha-2(I) chain; collagen alpha-2(I) chain; type I procollagen; alpha 2(I)-collagen; alpha-2 type I collagen; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-2 chain
UniProt Protein Name
Collagen alpha-2(I) chain
UniProt Synonym Protein Names
Alpha-2 type I collagen
UniProt Gene Name
COL1A2 [Similar Products]
UniProt Entry Name
CO1A2_HUMAN
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
