Product Name
GRIN2A, siRNA
Full Product Name
GRIN2A siRNA (Rat)
Product Synonym Names
Glutamate receptor ionotropic. NMDA 2A; GluN2A; Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A
Product Gene Name
GRIN2A sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q00959
Specificity
GRIN2A siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GRIN2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GRIN2A sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GRIN2A sirna
siRNA to inhibit GRIN2A expression using RNA interference
Applications Tested/Suitable for GRIN2A sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GRIN2A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036705.3
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NCBI GenBank Nucleotide #
NM_012573.3
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UniProt Primary Accession #
Q00959
[Other Products]
UniProt Secondary Accession #
O08948; Q63728[Other Products]
UniProt Related Accession #
Q00959[Other Products]
Molecular Weight
165,469 Da
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 2A
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, N-methyl D-aspartate 2A
NCBI Official Symbol
Grin2a [Similar Products]
NCBI Official Synonym Symbols
NR2A; GluN2A; NMDAR2A
[Similar Products]
NCBI Protein Information
glutamate receptor ionotropic, NMDA 2A
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 2A
UniProt Synonym Protein Names
Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A
Protein Family
Glutamate receptor ionotropic
UniProt Gene Name
Grin2a [Similar Products]
UniProt Synonym Gene Names
GluN2A; NMDAR2A; NR2A [Similar Products]
UniProt Entry Name
NMDE1_RAT
NCBI Summary for GRIN2A
may play a role in synaptic transmission, learning and memory [RGD, Feb 2006]
UniProt Comments for GRIN2A
NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission.
Protein type: Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass
Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; membrane; endoplasmic reticulum; postsynaptic density; dendritic spine; terminal button; synapse; cell junction; N-methyl-D-aspartate selective glutamate receptor complex
Molecular Function: voltage-gated cation channel activity; protein dimerization activity; neurotransmitter binding; glutamate receptor binding; zinc ion binding; calcium channel activity; protein kinase binding; ATPase binding; protein binding; extracellular-glutamate-gated ion channel activity; glutamate binding; protein heterodimerization activity; ionotropic glutamate receptor activity; N-methyl-D-aspartate selective glutamate receptor activity; protein complex binding; cell adhesion molecule binding; cation channel activity; receptor binding
Biological Process: regulation of long-term neuronal synaptic plasticity; startle response; positive regulation of apoptosis; rhythmic process; regulation of synaptic plasticity; response to other organism; sensory perception of pain; dopamine metabolic process; response to organic cyclic substance; response to carbohydrate stimulus; synaptic transmission; protein localization; learning and/or memory; calcium ion transport; response to methylmercury; response to wounding; visual learning; locomotion; serotonin metabolic process; protein tetramerization; cation transport; regulation of synaptic transmission; response to drug; regulation of action potential; synaptic transmission, glutamatergic; response to light stimulus; hippocampus development; response to amphetamine; learning; response to cocaine; sleep; memory; response to manganese ion; detection of mechanical stimulus involved in sensory perception of pain; regulation of membrane potential; response to ethanol; regulation of postsynaptic membrane potential; neurogenesis; spinal cord development; directional locomotion; ionotropic glutamate receptor signaling pathway; regulation of sensory perception of pain; cerebral cortex development; negative regulation of protein catabolic process; response to calcium ion; regulation of excitatory postsynaptic membrane potential; response to amine stimulus
Research Articles on GRIN2A
1. GluN1 amino-terminal domain adopts a more open conformation when coassembled with GluN2A than with GluN2B.
Precautions
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Disclaimer
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