Product Name
GRIN2A, siRNA
Full Product Name
GRIN2A siRNA (Mouse)
Product Synonym Names
Glutamate receptor ionotropic. NMDA 2A; GluN2A; Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A
Product Gene Name
GRIN2A sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P35436
Specificity
GRIN2A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GRIN2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GRIN2A sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GRIN2A sirna
siRNA to inhibit GRIN2A expression using RNA interference
Applications Tested/Suitable for GRIN2A sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GRIN2A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_032196.2
[Other Products]
NCBI GenBank Nucleotide #
NM_008170.2
[Other Products]
UniProt Primary Accession #
P35436
[Other Products]
UniProt Related Accession #
P35436[Other Products]
Molecular Weight
165,421 Da
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 2A
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, NMDA2A (epsilon 1)
NCBI Official Symbol
Grin2a [Similar Products]
NCBI Official Synonym Symbols
NR2A; GluN2A; NMDAR2A
[Similar Products]
NCBI Protein Information
glutamate receptor ionotropic, NMDA 2A
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 2A
UniProt Synonym Protein Names
Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A
Protein Family
Glutamate receptor ionotropic
UniProt Gene Name
Grin2a [Similar Products]
UniProt Synonym Gene Names
GluN2A; NMDAR2A; NR2A [Similar Products]
UniProt Entry Name
NMDE1_MOUSE
UniProt Comments for GRIN2A
NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission.
Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral
Cellular Component: neuron projection; cell surface; endoplasmic reticulum; postsynaptic density; dendritic spine; integral to membrane; terminal button; N-methyl-D-aspartate selective glutamate receptor complex; presynaptic membrane; postsynaptic membrane; synaptic vesicle; membrane; plasma membrane; synapse; cell junction
Molecular Function: protein dimerization activity; voltage-gated cation channel activity; neurotransmitter binding; glutamate receptor binding; zinc ion binding; metal ion binding; calcium channel activity; ion channel activity; receptor activity; protein kinase binding; ATPase binding; protein binding; glutamate binding; extracellular-glutamate-gated ion channel activity; protein heterodimerization activity; ionotropic glutamate receptor activity; N-methyl-D-aspartate selective glutamate receptor activity; protein complex binding; cell adhesion molecule binding; cation channel activity; receptor binding
Biological Process: regulation of long-term neuronal synaptic plasticity; startle response; positive regulation of apoptosis; rhythmic process; regulation of synaptic plasticity; locomotory behavior; sensory perception of pain; dopamine metabolic process; synaptic transmission; protein localization; learning and/or memory; transport; calcium ion transport; response to wounding; visual learning; locomotion; serotonin metabolic process; protein tetramerization; cation transport; regulation of synaptic transmission; response to drug; synaptic transmission, glutamatergic; regulation of action potential; response to amphetamine; learning; sleep; memory; detection of mechanical stimulus involved in sensory perception of pain; regulation of membrane potential; response to ethanol; regulation of postsynaptic membrane potential; neurogenesis; ionotropic glutamate receptor signaling pathway; directional locomotion; ion transport; regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; negative regulation of protein catabolic process
Research Articles on GRIN2A
1. We conclude that TSPYL2 contributes to cognitive variability through regulating the expression of Grin2a and Grin2b.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
