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Glycogen, Assay Kit

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产品名称: Glycogen, Assay Kit
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简单介绍

Glycogen, Assay Kit


Glycogen, Assay Kit  的详细介绍
Product Name

Glycogen (Gys1), Assay Kit

Popular Item
Full Product Name

Glycogen Colorimetric / Fluorometric Assay Kit

Product Gene Name

Gys1 assay kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
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Samples
Animal tissues: Liver, muscle etc.
Cell culture: Adherent or suspension cells.
Features & Benefits
Simple procedure; takes ~ 40 minutes
Fast and convenient
Kit contains all necessary reagents for accurate measurement of Glycogen
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Gys1 assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for
Gys1 assay kit
Product Information: Glycogen Assay Kit: Colorimetric & Fluorometric Assay for measuring Glycogen levels in a variety of Samples such as Tissue, Cells etc. within 30 min. Simple & Convenient. Detection Range: 0.0004 to 2 mg/ml. 100 Assays.

Background: Glycogen is the primary short term energy storage molecule in animals, synthesized primarily in the liver and muscle. Glycogen is a branched glucose polymer, in alpha-1,4 linkage, with branching via alpha-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases. The MyBioSource Kit is an easy and accurate assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a product that reacts with OxiRed probe to generate color (570 nm) and fluorescence (Ex 535/Em 587). The assay can detect glycogen 0.0004 to 2 mg/ml.

Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Applications- The assay can detect glycogen 0.0004 to 2 mg/ml.
Product Categories/Family for Gys1 assay kit
Diabetes, Obesity & Metabolic Syndrome; Signaling Pathways in Diabetes and Obesity; AMPK, Insulin and Leptin Signaling; Assay KitsSignaling Pathways; AMPK, Insulin and Leptin Signaling; Assay KitsMetabolism Assays; CarbohydratesMetabolism Assays; Metaboli

Typical Testing Data/Standard Curve (for reference only) of Gys1 assay kit
Gys1 assay kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for Gys1. It may not necessarily be applicable to this product.
NCBI GI #
157823922
NCBI GeneID
690987
NCBI Accession #
NP_001103085.1 [Other Products]
NCBI GenBank Nucleotide #
NM_001109615.1 [Other Products]
UniProt Related Accession #
A2RRU1[Other Products]
Molecular Weight
84,072 Da[Similar Products]
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NCBI Official Full Name
glycogen
NCBI Official Synonym Full Names
glycogen synthase 1, muscle
NCBI Official Symbol
Gys1  [Similar Products]
NCBI Protein Information
glycogen [starch] synthase, muscle
UniProt Protein Name
Glycogen [starch] synthase, muscle
Protein Family
Glycogenin
UniProt Gene Name
Gys1  [Similar Products]
UniProt Entry Name
GYS1_RAT
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UniProt Comments for Gys1
GYS1: muscle glycogen synthase 1. Transfers glucosyl residue from UDP-glucose to glycogen. Regulated allosterically by glucose-6-phosphate, and by PKA-mediated phosphorylation.

Protein type: EC 2.4.1.11; Transferase; Carbohydrate Metabolism - starch and sucrose

Cellular Component: membrane; cytoplasm; inclusion body

Molecular Function: glycogen (starch) synthase activity; protein kinase binding; glucose binding

Biological Process: glycogen metabolic process; glycogen biosynthetic process; heart development
Research Articles on Gys1
1. After contraction insulin-stimulated glycogen synthesis was increased by rather similar magnitude at all glycogen contents in concert with increased glycogen synthase activation.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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