ribonuclease, RNase A family, 7, ELISA Kit

Human Ribonuclease 7 (RNASE7) ELISA kit; MGC133220; ribonuclease 7; ribonuclease; RNase A family; 7

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of human RNASE7. No significant cross-reactivity or interference between human RNASE7 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of RNASE7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS922852 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ribonuclease, RNase A family, 7 (RNASE7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RNASE7. The ELISA analytical biochemical technique of the MBS922852 kit is based on RNASE7 antibody-RNASE7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RNASE7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RNASE7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RNASE7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RNASE7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNASE7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNASE7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

17,419 Da

ribonuclease, RNase A family, 7

ribonuclease 7; SAP-2; RNase 7; skin-derived antimicrobial protein 2

Ribonuclease 7

UNQ2516/PRO6006; RNase 7; SAP-2  [Similar Products]

RNAS7_HUMAN

RNASE7 is a member of the RNase A superfamily that has broad-spectrum antimicrobial activity and ribonuclease activity (Harder and Schroder, 2002 [PubMed 12244054]; Zhang et al., 2003 [PubMed 12527768]).[supplied by OMIM, Dec 2008]

RNASE7: Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium. Belongs to the pancreatic ribonuclease family.

Protein type: Secreted; RNA-binding; EC 3.1.27.-; Ribonuclease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular region

Molecular Function: peptidoglycan binding; nucleic acid binding; lipopolysaccharide binding; endonuclease activity; ribonuclease activity

Biological Process: defense response to Gram-positive bacterium; response to bacterium; antibacterial humoral response; innate immune response; membrane disruption in another organism; defense response to Gram-negative bacterium

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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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