Glutamate receptor ionotropic, NMDA 2A, Polyclonal Antibody

Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; GRIN2A; NMDAR2A

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Caprylic Acid Ammonium Sulfate Precipitation purified

Liquid

Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-GRIN2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Transport

ELISA (EIA), Immunohistochemistry (IHC)

Recommended dilution: IHC: 1:20-1:200

Immunohistochemistry of paraffin-embedded human pancreas using MBS7001732 at dilution 1:100

Western Blot
Positive WB detected in: U87 whole cell lysate,Rat heart tissue,Mouse liver tissue
All lanes: GRIN2A antibody at 3.2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 166,145 KDa
Observed band size: 166 KDa

Immunohistochemistry of paraffin-embedded human brain tissue at dilution of 1:100

Immunofluorescent analysis of HepG2 cells at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

144,431 Da

glutamate ionotropic receptor NMDA type subunit 2A

glutamate receptor ionotropic, NMDA 2A

Glutamate receptor ionotropic, NMDA 2A

NMDAR2A; GluN2A; NMDAR2A; NR2A; hNR2A  [Similar Products]

NMDE1_HUMAN

This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission.

Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p13.2

Cellular Component: cell junction; cell surface; endoplasmic reticulum; integral to plasma membrane; N-methyl-D-aspartate selective glutamate receptor complex; neuron projection; plasma membrane; postsynaptic membrane; presynaptic membrane; synaptic vesicle

Molecular Function: calcium channel activity; extracellular-glutamate-gated ion channel activity; N-methyl-D-aspartate selective glutamate receptor activity; protein binding; zinc ion binding

Biological Process: activation of MAPKK activity; axon guidance; directional locomotion; dopamine metabolic process; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; glutamate signaling pathway; innate immune response; insulin receptor signaling pathway; ionotropic glutamate receptor signaling pathway; learning and/or memory; MAPKKK cascade; memory; negative regulation of protein catabolic process; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; protein localization; Ras protein signal transduction; regulation of excitatory postsynaptic membrane potential; regulation of sensory perception of pain; response to amphetamine; response to drug; response to ethanol; response to wounding; sensory perception of pain; serotonin metabolic process; sleep; small GTPase mediated signal transduction; startle response; synaptic transmission; transport; vascular endothelial growth factor receptor signaling pathway; visual learning

Disease: Epilepsy, Focal, With Speech Disorder And With Or Without Mental Retardation

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