Aquaporin 4, Polyclonal Antibody

AQP4

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Affinity Purified

1mg/mL (lot specific)

-20 degree C

Small volumes of anti-Aqp4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background Info: Detects ~35kDa. May detect larger glycosylated bands ~50kDa.

Scientific Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 4 is constitutively expressed in the basolateral cell membrane of principal collecting duct cells in the kidney and provide a pathway for water to exit these cells (3). It is also expressed in astrocytes and is up regulated by direct insult to the CNS (4). Aquaporin 4 has been proposed as the primary autoimmune target in neuromyelitis optica (5, 6).

Transporters

Western Blot (WB), Immunofluorescence (IF)

1:1000-4000 (WB), 1:400 (IF)

Immunohistochemistry analysis using Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 um cryostat sections.

Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 4 protein using Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505). Primary Antibody: Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505) at 1:2000.

28,768 Da[Similar Products]

aquaporin 4

aquaporin-4; WCH4; mercurial-insensitive water channel

Aquaporin-4

AQP-4; MIWC  [Similar Products]

AQP4_RAT

The protein encoded by this gene forms a water-specific channel that regulates body water balance, particularly in the brain. The encoded protein acts as a membrane-bound homotetramer, interacting with other proteins (MLC1, TRPV4, HEPACAM, and ATP1B1) to form a complex. Several variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

AQP4: a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The predominant aquaporin found in brain. Two alternatively spliced transcript variants have been described

Protein type: Transporter, aquaporin family; Membrane protein, multi-pass; Membrane protein, integral; Channel, misc.; Transporter

Cellular Component: protein complex; membrane; cell projection membrane; integral to plasma membrane; basolateral plasma membrane; T-tubule; cytoplasm; plasma membrane; basal plasma membrane; intercellular junction; sarcolemma; external side of plasma membrane

Molecular Function: water channel activity; porin activity; glycerol channel activity

Biological Process: glycerol transport; cellular water homeostasis; carbon dioxide transport; water transport; response to glucocorticoid stimulus; negative regulation of interleukin-1 beta production; female pregnancy; water homeostasis; response to radiation; sensory perception of sound; hyperosmotic salinity response; negative regulation of interleukin-6 production; multicellular organismal water homeostasis; transmembrane transport; protein homooligomerization

1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396. 2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258. 3. Agre P., Nielsen S. (1996) Nephrologie. 17(7): 409-415. 4. Nagelhus E.A., Mathiisen T.M., Ottersen O.P. (2004) Neuroscience. 129(4): 905-913. 5. Lennon V.A., Kryzer T.J., Pittock S.J., Verkman A.S., Hinson S.R. (2005) J Exp Med. 202(4): 473- 477. 6. Bradl M., Lassmann D.H. (2008) Int. MS Journal/ MS Forum. 15(3): 75-78.

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