Full Product Name
CPOX siRNA (Mouse)
Product Synonym Names
CPO; Oxygen-dependent coproporphyrinogen-III oxidase mitochondrial; COX; Coprogen oxidase; Coproporphyrinogenase
Product Gene Name
CPOX sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P36552
Specificity
CPOX siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CPOX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CPOX sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CPOX sirna
siRNA to inhibit CPOX expression using RNA interference
Applications Tested/Suitable for CPOX sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CPOX. It may not necessarily be applicable to this product.
NCBI Accession #
NP_031783.2
[Other Products]
NCBI GenBank Nucleotide #
NM_007757.2
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UniProt Primary Accession #
P36552
[Other Products]
UniProt Secondary Accession #
Q7TQ36; Q8VD08[Other Products]
UniProt Related Accession #
P36552[Other Products]
Molecular Weight
49,715 Da
NCBI Official Full Name
oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
NCBI Official Synonym Full Names
coproporphyrinogen oxidase
NCBI Official Symbol
Cpox [Similar Products]
NCBI Official Synonym Symbols
CPX; Cpo; HCP; cac; nct
[Similar Products]
NCBI Protein Information
oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
UniProt Protein Name
Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
Protein Family
Oxygen-dependent coproporphyrinogen-III oxidase
UniProt Gene Name
Cpox [Similar Products]
UniProt Synonym Gene Names
Cpo; COX; Coprogen oxidase; Coproporphyrinogenase [Similar Products]
UniProt Entry Name
HEM6_MOUSE
UniProt Comments for CPOX
CPOX: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III. Defects in CPOX are the cause of hereditary coproporphyria (HCP). HCP is an acute hepatic porphyria and an autosomal dominant disease characterized by neuropsychiatric disturbances and skin photosensitivity. Biochemically, there is an overexcretion of coproporphyrin III in the urine and in the feces. HCP is clinically characterized by attacks of abdominal pain, neurological disturbances, and psychiatric symptoms. The symptoms are generally manifested with rapid onset, and can be precipitated by drugs, alcohol, caloric deprivation, infection, endocrine factors or stress. A severe variant form is harderoporphyria, which is characterized by earlier onset attacks, massive excretion of harderoporphyrin in the feces, and a marked decrease of coproporphyrinogen IX oxidase activity. Belongs to the aerobic coproporphyrinogen-III oxidase family.
Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Oxidoreductase; EC 1.3.3.3; Mitochondrial
Cellular Component: mitochondrion; membrane; cytoplasm; mitochondrial inner membrane; mitochondrial intermembrane space
Molecular Function: coproporphyrinogen oxidase activity; identical protein binding; protein homodimerization activity; sodium:potassium-exchanging ATPase activity; oxidoreductase activity; structural constituent of eye lens
Biological Process: sodium ion transport; porphyrin biosynthetic process; protoporphyrinogen IX biosynthetic process; potassium ion transport; heme biosynthetic process
Research Articles on CPOX
1. The mechanisms by which impairment of CPOX leads to lens opacity in the NCT are elusive. However, our data illuminate a hitherto unanticipated involvement of the heme biosynthesis pathway in lens physiology.
Precautions
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Disclaimer
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