Full Product Name
CPOX antibody
Product Synonym Names
Coproporphyrinogen-III oxidase mitochondrial precursor; Coproporphyrinogen-III oxidase; mitochondrial precursor; CPO; CPX; HCP
Product Gene Name
anti-CPOX antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P36551
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (lot specific)
Immunogen
Recombinant human CPOX (111-454aa) purified from E. coli
Clone Note
Anti-human CPOX mAb, clone AT36B10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CPOX protein
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-CPOX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CPOX antibody
Coproporphyrinogen-III oxidase, mitochondrial precursor, also known as CPOX, is a 454 amino acid mitochondrial enzyme that is localized to the inner membrane space of erythrocytes. It participates in the sixth step of heme biosynthesis by catalyzing the formation of protoporphyrinogen IX from coproporphyrinogen III. Mutations in human CPOX gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.
Product Categories/Family for anti-CPOX antibody
Signal Transduction; Metabolism
Applications Tested/Suitable for anti-CPOX antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Western Blot (WB) of anti-CPOX antibody
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CPOX antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant protein CPOX Lane 2.: 293T cell lysate Lane 3.: HepG2 cell lysate Lane 4.: A549 cell lysate Lane 5.: Jurkat cell lysate Lane 6.: K562 cell lysate Lane 7.: LnCaP cell lysate
Immunofluorescence (IF) of anti-CPOX antibody
ICC/IF analysis of CPOX in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CPOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
NCBI/Uniprot data below describe general gene information for CPOX. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000088
[Other Products]
NCBI GenBank Nucleotide #
NM_000097.5
[Other Products]
UniProt Primary Accession #
P36551
[Other Products]
UniProt Secondary Accession #
Q14060; Q53F08; Q8IZ45; Q96AF3; A8K275; B4DSD5[Other Products]
UniProt Related Accession #
P36551[Other Products]
Molecular Weight
30,383 Da
NCBI Official Full Name
oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
NCBI Official Synonym Full Names
coproporphyrinogen oxidase
NCBI Official Symbol
CPOX [Similar Products]
NCBI Official Synonym Symbols
CPO; CPX; HCP
[Similar Products]
NCBI Protein Information
oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
UniProt Protein Name
Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
Protein Family
Oxygen-dependent coproporphyrinogen-III oxidase
UniProt Gene Name
CPOX [Similar Products]
UniProt Synonym Gene Names
CPO; CPX; COX; Coprogen oxidase; Coproporphyrinogenase [Similar Products]
NCBI Summary for CPOX
The protein encoded by this gene is the sixth enzyme of the heme biosynthetic pathway. The encoded enzyme is soluble and found in the intermembrane space of mitochondria. This enzyme catalyzes the stepwise oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX, a precursor of heme. Defects in this gene are a cause of hereditary coproporphyria (HCP).[provided by RefSeq, Oct 2009]
UniProt Comments for CPOX
Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.
Product References and Citations for anti-CPOX antibody
Kim DH., et al. (2013) J Biochem. 154 (6): 551-9.; Barbaro M., et al. (2012) Clin Genet. 81 (3): 249-56.; Rosipal R., et al. (1999) Hum Mutat. 13 (1): 44-53.;
Research Articles on CPOX
1. The monomer form of mutated CPOX did not show any activity and homodimeric enzymes derived from Hereditary coproporphyria (HCP) mutant showed low activity (<20% of the control).
Precautions
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Disclaimer
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