Full Product Name
CD71 (Phospho-Tyr20) Antibody
Product Gene Name
anti-CD71 antibody
[Similar Products]
Product Synonym Gene Name
TFRC[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P02786
Species Reactivity
Human, Mouse
Specificity
CD71 (Phospho-Tyr20) antibody detects endogenous levels of CD71 only when phosphorylated at Tyr20.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Phospho
Modification Sites
H:Y20; M:Y20
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human CD71 around the phosphorylation site of Tyr20.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-CD71 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD71 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-CD71 antibody
WB: 1:500~1:1000
ELISA: 1:10000
ELISA (EIA) of anti-CD71 antibody
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD71 (Phospho-Tyr20) Antibody
Western Blot (WB) of anti-CD71 antibody
Western blot analysis of lysates from 293 cells treated with PMA, using phospho-CD71 (Phospho-Tyr20) antibody.
NCBI/Uniprot data below describe general gene information for CD71. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001121620.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001128148.2
[Other Products]
UniProt Primary Accession #
P02786
[Other Products]
UniProt Secondary Accession #
Q1HE24; Q59G55; Q9UCN0; Q9UCU5; Q9UDF9; Q9UK21; D3DXB0[Other Products]
UniProt Related Accession #
P02786[Other Products]
NCBI Official Full Name
transferrin receptor protein 1 isoform 1
NCBI Official Synonym Full Names
transferrin receptor
NCBI Official Symbol
TFRC [Similar Products]
NCBI Official Synonym Symbols
T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46
[Similar Products]
NCBI Protein Information
transferrin receptor protein 1
UniProt Protein Name
Transferrin receptor protein 1
UniProt Synonym Protein Names
T9; p90; CD_antigen: CD71
UniProt Gene Name
TFRC [Similar Products]
UniProt Synonym Gene Names
TR; TfR; TfR1; Trfr; sTfR [Similar Products]
NCBI Summary for CD71
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
UniProt Comments for CD71
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal . Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
Research Articles on CD71
1. results suggest that lower Hepcidin-25, as well as higher sTfR and sTfR/Hepcidin-25 ratio were significant predictors of favorable hemoglobin response within a month after IV administration of ferric carboxymaltose in patients with CKD
Precautions
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Disclaimer
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