Product Name
ACTA1, siRNA
Full Product Name
ACTA1 siRNA (Rat)
Product Synonym Names
ACTA; Actin alpha skeletal muscle; Alpha-actin-1
Product Gene Name
ACTA1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P68136
Specificity
ACTA1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ACTA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ACTA1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ACTA1 sirna
siRNA to inhibit ACTA1 expression using RNA interference
Applications Tested/Suitable for ACTA1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ACTA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_062085.1
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NCBI GenBank Nucleotide #
NM_019212.2
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UniProt Primary Accession #
P68136
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UniProt Secondary Accession #
P02568; P99020[Other Products]
UniProt Related Accession #
P68136[Other Products]
Molecular Weight
42,051 Da
NCBI Official Full Name
actin, alpha skeletal muscle
NCBI Official Synonym Full Names
actin, alpha 1, skeletal muscle
NCBI Official Symbol
Acta1 [Similar Products]
NCBI Protein Information
actin, alpha skeletal muscle
UniProt Protein Name
Actin, alpha skeletal muscle
UniProt Synonym Protein Names
Alpha-actin-1
UniProt Gene Name
Acta1 [Similar Products]
UniProt Synonym Gene Names
Acta [Similar Products]
UniProt Entry Name
ACTS_RAT
NCBI Summary for ACTA1
may act as a structural component of the cytoskeleton; may play a role in muscle function [RGD, Feb 2006]
UniProt Comments for ACTA1
ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family.
Protein type: Contractile; Cell development/differentiation; Motility/polarity/chemotaxis; Cytoskeletal
Cellular Component: extracellular space; sarcomere; stress fiber; actin filament; striated muscle thin filament; actin cytoskeleton
Molecular Function: ATP binding
Biological Process: skeletal muscle fiber adaptation; response to steroid hormone stimulus; response to mechanical stimulus; muscle thin filament assembly; response to lithium ion; response to extracellular stimulus; skeletal muscle fiber development; cell growth
Research Articles on ACTA1
1. analysis of the pattern of of evolutionarily conserved basic and acidic residues that constitutes the binding interface of actin-tropomyosin
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
