NOTCH3, Polyclonal Antibody

NOTCH3; Notch homolog 3 (Drosophila); HGNC: 7883; CADASIL; CASIL; Notch (Drosophila) homolog 3; Notch homolog 3; NOTCH3 antibody; Notch homolog 3 (Drosophila) antibody; HGNC: 7883 antibody; CADASIL antibody; CASIL antibody; Notch (Drosophila) homolog 3 antibody; Notch homolog 3 antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-NOTCH3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Hippocampus shows select nuclear staining and weaker staining in different comparments of the neuronal cell bodies. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa according to NP_000426.1. The 60+70kDa bands were successfully blocked by incubation with the immunizing peptide.

(4ug/ml) staining of paraffin embedded Human Hippocampus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

243,631 Da

notch 3

neurogenic locus notch homolog protein 3

Neurogenic locus notch homolog protein 3

Notch 3  [Similar Products]

NOTC3_HUMAN

This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq, Jul 2008]

NOTCH3: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH3. Interacts with PSMA1. Interacts with HIF1AN. Ubiquitously expressed in fetal and ***** tissues. Belongs to the NOTCH family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2-p13.1

Cellular Component: actin cytoskeleton; cytoplasm; cytosol; endoplasmic reticulum membrane; extracellular region; Golgi membrane; integral to membrane; nucleoplasm; plasma membrane; receptor complex

Molecular Function: calcium ion binding; enzyme binding; protein binding

Biological Process: artery morphogenesis; forebrain development; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; neuron fate commitment; Notch receptor processing; Notch signaling pathway; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter

Disease: Cerebral Arteriopathy, Autosomal Dominant, With Subcortical Infarcts And Leukoencephalopathy; Lateral Meningocele Syndrome; Myofibromatosis, Infantile, 2

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