ACTA1, Polyclonal Antibody

ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total ACTA1 protein.

Antigen affinity purification.

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

2.3 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-ACTA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC)

Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200

Gel: 10%SDS-PAGE Lysates (from left to right): Mouse muscle and heart tissue Amount of lysate: 40ug per lane Primary antibody: 1/500 dilution Secondary antibody dilution: 1/8000 Exposure time: 20 seconds

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using at dilution 1/65.

42,051 Da

actin, alpha 1, skeletal muscle

actin, alpha skeletal muscle

Actin, alpha skeletal muscle

ACTA  [Similar Products]

ACTS_HUMAN

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family.

Protein type: Motility/polarity/chemotaxis; Cell development/differentiation; Cytoskeletal; Contractile

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: extracellular space; sarcomere; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton

Molecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding

Biological Process: skeletal muscle fiber adaptation; muscle contraction; muscle thin filament assembly; response to mechanical stimulus; response to steroid hormone stimulus; response to lithium ion; response to extracellular stimulus; skeletal muscle fiber development; cell growth; muscle filament sliding

Disease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion

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