ACTA1, Monoclonal Antibody

Actin; alpha skeletal muscle; Alpha-actin-1; ACTA1; ACTA

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1,k

337CT30.10.1

Mouse

This ACTA1 monoclonal antibody is generated from mouse immunized with ACTA1 recombinant protein.

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-ACTA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The product encoded by this gene belongs to the actin
family of proteins, which are highly conserved proteins that play a
role in cell motility, structure and integrity. Alpha, beta and
gamma actin isoforms have been identified, with alpha actins being
a major constituent of the contractile apparatus, while beta and
gamma actins are involved in the regulation of cell motility. This
actin is an alpha actin that is found in skeletal muscle. Mutations
in this gene cause nemaline myopathy type 3, congenital myopathy
with excess of thin myofilaments, congenital myopathy with cores,
and congenital myopathy with fiber-type disproportion, diseases
that lead to muscle fiber defects.

Cancer; Cardiovascular; Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

WB~~1:100~500
IHC~~1:10~50
FC~~1:10~50

ACTA1 Antibody western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ACTA1 antibody detected the ACTA1 protein (arrow).

ACTA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

ACTA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

ACTA1 Antibody flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

42051

actin, alpha 1, skeletal muscle

actin, alpha skeletal muscle

Actin, alpha skeletal muscle

ACTA  [Similar Products]

ACTS_HUMAN

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Contractile; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: extracellular space; sarcomere; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton

Molecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding

Biological Process: skeletal muscle fiber adaptation; muscle contraction; muscle thin filament assembly; response to mechanical stimulus; response to steroid hormone stimulus; response to lithium ion; response to extracellular stimulus; skeletal muscle fiber development; cell growth; muscle filament sliding

Disease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion

Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010) :
Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010)
Yu, G., et al. J Clin Neurosci 17(6):766-769(2010)
Yu, C.H., et al. PLoS ONE 5 (7), E11878 (2010) :
Licastro, F., et al. Curr. Pharm. Des. 16(7):783-788(2010)

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