Histone-lysine N-methyltransferase EHMT1 (EHMT1), ELISA Kit

RP11-188C12.1; DEL9q34; DKFZp667M072; EUHMTASE1; Eu-HMTase1; FLJ12879; FP13812; GLP; KIAA1876; KMT1D; bA188C12.1; G9a like protein; H3-K9-HMTase 5; histone H3-K9 methyltransferase 5; histone-lysine N-m

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse EHMT1. No significant cross-reactivity or interference between Mouse EHMT1 and analogues was observed.

Store at 2-8 degree C.

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Small volumes of EHMT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS287696 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Histone-lysine N-methyltransferase EHMT1 (EHMT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EHMT1. The ELISA analytical biochemical technique of the MBS287696 kit is based on EHMT1 antibody-EHMT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EHMT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EHMT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: For the quantitative detection of Mouse Histone-lysine N-methyltransferase EHMT1 (EHMT1) concentration in serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate EHMT1 in Mouse serum, plasma, tissue homogenates. An antibody specific for EHMT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EHMT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EHMT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EHMT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: EHMT1 is a histone methyltransferase that is part of the E2F6 complex, which represses transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. The 1,267-amino acid EU-HMTase-1 protein contains ankyrin repeats and a SET domain. EU-HMTase-1 shares 63% sequence similarity with NG36/G9A. EU-HMTase-1 exhibited histone methyltransferase activity with a specificity for histone H3 in core histones, whereas it hardly methylated nucleosomes, suggesting that other subunits in the E2F6 complex are required for modification of nucleosomal substrates.

136,631 Da

euchromatic histone methyltransferase 1

histone-lysine N-methyltransferase EHMT1

Histone-lysine N-methyltransferase EHMT1

Euhmtase1; Glp; Kmt1d; Eu-HMTase1; GLP; GLP1  [Similar Products]

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.

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